Human IGFBP7 ELISA Kit (ab229894)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 7.6 pg/ml
- Range: 54.69 pg/ml - 3500 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cell Lysate, Cit plasma, EDTA Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IGFBP7 ELISA Kit
See all IGFBP7 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Human serum 5 2.7% Inter-assay Sample n Mean SD CV% Human serum 3 2.6% -
Sample type
Cell culture supernatant, Urine, Serum, Cell culture extracts, Cell Lysate, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
7.6 pg/ml -
Range
54.69 pg/ml - 3500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 98 86% - 111% Urine 104 92% - 114% Serum 107 94% - 115% Cell culture extracts 80 70% - 88% EDTA Plasma 120 115% - 122% Cit plasma 98 83% - 107% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human IGFBP7 ELISA Kit (ab229894) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IGFBP7 protein in cell culture supernatant, cit plasma, edta plasma, serum, urine, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IGFBP7 with 7.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IGFBP7 Capture Antibody 1 x 600µl 10X Human IGFBP7 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human IGFBP7 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 Kazal-like domain. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- AGM
- Angiomodulin
- FSTL2
see all -
Database links
- Entrez Gene: 3490 Human
- Omim: 602867 Human
- SwissProt: Q16270 Human
- Unigene: 479808 Human
Images
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Other - Human IGFBP7 ELISA Kit (ab229894)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human IGFBP7 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of human IGFBP7 standard curve in 1X Cell Extraction Buffer PTRBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native IGFBP7 in human serum and plasma samples.The concentrations of IGFBP7 were measured in duplicates, interpolated from the IGFBP7 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1%, plasma (citrate) 1%, and plasma (EDTA) 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP7 concentration was determined to be 205.0 ng/mL in neat serum, 196.4 ng/mL in neat plasma (citrate) and 189.9 ng/mL in neat plasma (EDTA).
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Interpolated concentrations of native IGFBP7 in human urine and cell culture supernatant samples.The concentrations of IGFBP7 were measured in duplicates, interpolated from the IGFBP7 standard curves and corrected for sample dilution. Undiluted samples are as follows: male urine 0.5%, female urine 0.5%, and HeLa cell culture supernatant 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP7 concentration was determined to be 337.7 ng/mL in neat male urine, 397.6 ng/mL in neat female urine, and 389.5 ng/mL in neat HeLa cell culture supernatant.
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Interpolated concentrations of native IGFBP7 in HeLa cell extract based on a 20 µg/mL extract load.The concentrations of IGFBP7 were measured in duplicate and interpolated from the IGFBP7 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP7 concentration was determined to be 2884 pg/mL in HeLa cell extract.
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP7 concentration was determined to be 233.5 ng/mL with a range of 180.8 – 335.8 ng/mL.
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Sandwich ELISA - Human IGFBP7 ELISA Kit (ab229894)To learn more about the advantages of recombinant antibodies see here.
