Human IGFBP2 ELISA Kit (ab215082)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 480 pg/ml
- Range: 1.1 ng/ml - 70 ng/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human IGFBP2 ELISA Kit
See all IGFBP2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 3 4.9% Inter-assay Sample n Mean SD CV% Overall 5 4.5% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
480 pg/ml -
Range
1.1 ng/ml - 70 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 80 79% - 81% Urine 99 99% - 100% Serum 105 101% - 109% Hep Plasma 112 106% - 121% EDTA Plasma 107 105% - 108% Cit plasma 114 103% - 126% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Goat, Cow -
Product overview
Human IGFBP2 ELISA kit has been re-developed. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency. This version will be discontinued October 2020 unless inventory is depleted beforehand. The new verison is available as ab272207.
Human IGFBP2 ELISA Kit (ab215082) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IGFBP2 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IGFBP2 with 480 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Notes
Insulin-like growth factor-binding protein 2 (IGFBP2) is an IGF binding protein involved in prolonging its half-life, and has both inhibitory and stimulatory effects on IGFs. IGFBP2 can also alter the interaction of IGFs with their receptors.
-
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IGFBP2 Capture Antibody 1 x 600µl 10X Human IGFBP2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human IGFBP2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. -
Sequence similarities
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
Domain
The C-terminus is required for IGF-binding and growth inhibition. -
Post-translational
modificationsO-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- BP 2
- BP2
- IBP 2
see all -
Database links
- Entrez Gene: 3485 Human
- Omim: 146731 Human
- SwissProt: P18065 Human
- Unigene: 438102 Human
Images
-
Other - Human IGFBP2 ELISA Kit (ab215082)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of human IGFBP2 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of native IGFBP2 in human serum and plasma samples.The concentrations of IGFBP2 were measured in duplicates, interpolated from the IGFBP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (heparin) 25% and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP2 concentration was determined to be 260 ng/mL in serum, 145 ng/mL in plasma (citrate), 217 ng/mL in plasma (EDTA), and 140ng/mL in plasma (heparin).
-
Serum from ten individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP2 concentration was determined to be 112 ng/mL with a range of 32 – 219 ng/mL.
