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Signal Transduction Growth Factors/Hormones Insulin / Insulin-like

Rat Serpin A12  Antibody Pair - BSA and Azide free (ab253333)

Rat Serpin A12  Antibody Pair - BSA and Azide free (ab253333)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Rat

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Overview

  • Product name

    Rat Serpin A12  Antibody Pair - BSA and Azide free
    See all Vaspin kits
  • Assay type

    ELISA set
  • Range

    23.438 pg/ml - 1500 pg/ml
  • Species reactivity

    Reacts with: Rat
  • Product overview

    The Antibody Pair can be used to quantify Rat Serpin A12. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab272197), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Carrier free

    Yes
  • Components 10 x 96 tests
    Rat Serpin A12 Capture Antibody (unconjugated) 1 x 100µg
    Rat Serpin A12 Detector Antibody (unconjugated) 1 x 100µg
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • Insulin / Insulin-like
    • Neuroscience
    • Neurology process
    • Metabolism
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Protease inhibitors
    • Serine protease inhibitors
    • SERPINs
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Protease inhibitors
    • Other protease inhibitors
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Protease inhibitors ELISA kits
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Heart disease
  • Function

    May modulates insulin action conceivably only in the presence of its yet undefined target proteases in white adipose tissues.
  • Tissue specificity

    Expressed in visceral adipose tissues.
  • Sequence similarities

    Belongs to the serpin family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q8IW75 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • ENSG00000165953
    • OL 64
    • OL-64
    • OL64
    • Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 12
    • Serpin A12
    • Serpin A12 precursor
    • Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 12
    • Serpina12
    • SPA12_HUMAN
    • Vaspin
    • Visceral adipose specific serpin
    • Visceral adipose tissue derived serine protease inhibitor
    • Visceral adipose tissue-derived serine protease inhibitor
    • Visceral adipose-specific serpin
    see all
  • Database links

    • Entrez Gene: 191570 Rat
    • SwissProt: Q8R4Z1 Rat
    • Unigene: 79035 Rat

    Images

    • Sandwich ELISA - Rat Serpin A12  Antibody Pair - BSA and Azide free (ab253333)
      Sandwich ELISA - Rat Serpin A12  Antibody Pair - BSA and Azide free (ab253333)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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