Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µl 103 862 ₸

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Anti-Vaspin antibody [EPR23080-232] (ab267471)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23080-232] to Vaspin
Suitable for: IP, WB
Reacts with: Rat, Human

Product name

Anti-Vaspin antibody [EPR23080-232]
See all Vaspin primary antibodies
Description

Rabbit monoclonal [EPR23080-232] to Vaspin
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Rat

WB
Rat

Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Rat stomach and Rat skin lysate; His-tagged recombinant Rat Serpin A12 Protein. IP: Rat stomach tissue lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Rat
WB	Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23080-232
Isotype

IgG
Research areas

Western blot - Anti-Vaspin antibody [EPR23080-232] (ab267471)

All lanes : Anti-Vaspin antibody [EPR23080-232] (ab267471) at 1/1000 dilution

Lane 1 : Rat stomach tissue lysate at 20 µg
Lane 2 : Rat kidney tissue lysate at 20 µg
Lane 3 : Rat skin tissue lysate at 60 µg

Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 47 kDa
Observed band size: 42,47 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The band of 42kDa is the endogenously cleaved form.

The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 19554505, 16030142, 26199422).

Negative control: kidney (PMID: 19554505).

Exposure time: lane1-2: 59 seconds lane 3: 3 minutes
Immunoprecipitation - Anti-Vaspin antibody [EPR23080-232] (ab267471)

Vaspin was immunoprecipitated from 0.35 mg Rat stomach tissue lysate with ab267471 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab267471 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Rat stomach tissue lysate 10ug

Lane 2: ab267471 IP in Rat stomach tissue lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab267471 in Rat stomach tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes
Western blot - Anti-Vaspin antibody [EPR23080-232] (ab267471)

Anti-Vaspin antibody [EPR23080-232] (ab267471) at 1/1000 dilution + His-tagged recombinant Rat Serpin A12 Protein (aa20-411),

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 47 kDa
Observed band size: 47 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Exposure time: 26 seconds
Anti-Vaspin antibody [EPR23080-232] (ab267471)

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