Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Vaspin antibody [EPR23080-232] (ab267471)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23080-232] to Vaspin
  • Suitable for: IP, WB
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-Vaspin antibody [EPR23080-232]
    See all Vaspin primary antibodies

  • Description

    Rabbit monoclonal [EPR23080-232] to Vaspin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Rat
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Rat stomach and Rat skin lysate; His-tagged recombinant Rat Serpin A12 Protein. IP: Rat stomach tissue lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Vaspin antibody [EPR23080-232] (ab267471)
    Western blot - Anti-Vaspin antibody [EPR23080-232] (ab267471)
    All lanes : Anti-Vaspin antibody [EPR23080-232] (ab267471) at 1/1000 dilution

    Lane 1 : Rat stomach tissue lysate at 20 µg
    Lane 2 : Rat kidney tissue lysate at 20 µg
    Lane 3 : Rat skin tissue lysate at 60 µg

    Secondary
    Lanes 1-2 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
    Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 47 kDa
    Observed band size: 42,47 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The band of 42kDa is the endogenously cleaved form.

    The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 19554505, 16030142, 26199422).

    Negative control: kidney (PMID: 19554505).

    Exposure time: lane1-2: 59 seconds lane 3: 3 minutes

  • Immunoprecipitation - Anti-Vaspin antibody [EPR23080-232] (ab267471)
    Immunoprecipitation - Anti-Vaspin antibody [EPR23080-232] (ab267471)

    Vaspin was immunoprecipitated from 0.35 mg Rat stomach tissue lysate with ab267471 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab267471 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Rat stomach tissue lysate 10ug

    Lane 2: ab267471 IP in Rat stomach tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab267471 in Rat stomach tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes

     

  • Western blot - Anti-Vaspin antibody [EPR23080-232] (ab267471)
    Western blot - Anti-Vaspin antibody [EPR23080-232] (ab267471)
    Anti-Vaspin antibody [EPR23080-232] (ab267471) at 1/1000 dilution + His-tagged recombinant Rat Serpin A12 Protein (aa20-411),

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 47 kDa
    Observed band size: 47 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 26 seconds

  • Anti-Vaspin antibody [EPR23080-232] (ab267471)
    Anti-Vaspin antibody [EPR23080-232] (ab267471)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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