Mouse Insulin like Growth Factor 1 ELISA kit (IGF1) (ab108874)
Key features and details
- Sensitivity: 0.12 ng/ml
- Range: 0.3 ng/ml - 5 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
-
Product name
Mouse Insulin like Growth Factor 1 ELISA kit (IGF1)
See all IGF1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 3.9% Inter-assay Sample n Mean SD CV% Overall 8.6% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
= 0.12 ng/ml -
Range
0.3 ng/ml - 5 ng/ml -
Recovery
96 %
-
Assay time
5h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s Insulin like Growth Factor 1 (IGF1) Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Insulin like Growth Factor 1 levels in plasma, serum and cell culture supernatants.
An IGF1 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IGF1 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IGF1 captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
-
Platform
Microplate
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 40X Biotinylated Mouse Insulin like Growth Factor 1 Antibody 1 x 150µl Chromogen Substrate 1 x 7ml Insulin like Growth Factor 1 Microplate (12 x 8 well strips) 1 unit Insulin like Growth Factor 1 Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
Research areas
-
Function
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. -
Involvement in disease
Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. -
Sequence similarities
Belongs to the insulin family. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- IBP1
- IGF I
- IGF IA
see all -
Database links
- Entrez Gene: 16000 Mouse
- SwissProt: P05017 Mouse
- Unigene: 268521 Mouse
Images
-
Typical Standard Curve
Representative Standard Curve using ab108874
