Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Proinsulin antibody [3B6-19-1] - BSA and Azide free (ab255745)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [3B6-19-1] to Proinsulin - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, Dot blot
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Proinsulin antibody [3B6-19-1] - BSA and Azide free
    See all Proinsulin primary antibodies

  • Description

    Mouse monoclonal [3B6-19-1] to Proinsulin - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, Dot blotmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: Human PBMC.IHC-P: Mouse, rat and human pancreas tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255745 is the carrier-free version of ab243141. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proinsulin antibody [3B6-19-1] - BSA and Azide free (ab255745)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proinsulin antibody [3B6-19-1] - BSA and Azide free (ab255745)

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling Proinsulin with ab243141 at 0.49 µg/mL, followed by a ready to use secondary. Positive staining on islets in the rat pancreas is observed. The section was incubated with ab243141 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument with no antigen retrieval. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by a ready to use secondary.

    This image was produced using the same antibody clone but in a different formulation ab243141, PBS, sodium azide, glycerol and BSA.

  • Flow Cytometry - Anti-Proinsulin antibody [3B6-19-1] - BSA and Azide free (ab255745)
    Flow Cytometry - Anti-Proinsulin antibody [3B6-19-1] - BSA and Azide free (ab255745)

    Flow cytometric analysis of 2% PFA-fixed, 0.1% Tween-20 permeabilized human peripheral blood mononuclear cell (PBMC) (right) labeling Proinsulin with ab243141 at 0.98 µg/mL compared with mouse monoclonal IgG Isotype Control (left). Goat anti-mouse IgG (Alexa Fluor® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with mouse IgG (Left) or ab243141 (Right). Then stained with anti-CD14 (Alexa Fluor® 647).

    Gated on viable cells.

    This image was produced using the same antibody clone but in a different formulation ab243141, PBS, sodium azide, glycerol and BSA.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proinsulin antibody [3B6-19-1] - BSA and Azide free (ab255745)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proinsulin antibody [3B6-19-1] - BSA and Azide free (ab255745)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Proinsulin with ab243141 at 0.49 µg/mL, followed by a ready to use secondary. Positive staining on islets in the human pancreas is observed. The section was incubated with ab243141 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument with no antigen retrieval. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by a ready to use secondary.

    This image was produced using the same antibody clone but in a different formulation ab243141, PBS, sodium azide, glycerol and BSA.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proinsulin antibody [3B6-19-1] - BSA and Azide free (ab255745)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proinsulin antibody [3B6-19-1] - BSA and Azide free (ab255745)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling Proinsulin with ab243141 at 0.49 µg/mL, followed by a ready to use secondary. Positive staining on islets in the mouse pancreas is observed. The section was incubated with ab243141 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument with no antigen retrieval. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by a ready to use secondary.

    This image was produced using the same antibody clone but in a different formulation ab243141, PBS, sodium azide, glycerol and BSA.

  • Dot Blot - Anti-Proinsulin antibody [3B6-19-1] - BSA and Azide free (ab255745)
    Dot Blot - Anti-Proinsulin antibody [3B6-19-1] - BSA and Azide free (ab255745)

    Dot Blot - Anti-Proinsulin antibody [3B6-19-1] (ab243141, 0.49 µg/mL)

    Secondary used is a Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution.

    Sample is an insulin recombinant protein.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This image was produced using the same antibody clone but in a different formulation ab243141, PBS, sodium azide, glycerol and BSA.

  • Anti-Proinsulin antibody [3B6-19-1] - BSA and Azide free (ab255745)
    Anti-Proinsulin antibody [3B6-19-1] - BSA and Azide free (ab255745)

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