Anti-BubR1 (phospho T680) antibody [EPR19958] - BSA and Azide free (ab251297)
![Anti-BubR1 (phospho T680) antibody [EPR19958] - BSA and Azide free (ab251297)](https://www.abcam.com/ps/products/251/ab251297/Images/ab251297-4-anti-bubr1-phospho-t680-antibody-epr19958-bsa-and-azide-free-immunocytochemistry.jpg "Anti-BubR1 (phospho T680) antibody [EPR19958] - BSA and Azide free (ab251297)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19958] to BubR1 (phospho T680) - BSA and Azide free
- Suitable for: Flow Cyt, ICC, WB, IP
- Reacts with: Human
Overview
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Product name
Anti-BubR1 (phospho T680) antibody [EPR19958] - BSA and Azide free
See all BubR1 primary antibodies -
Description
Rabbit monoclonal [EPR19958] to BubR1 (phospho T680) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, ICC, WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251297 is the carrier-free version of ab200061. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251297 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19958 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry - Anti-BubR1 (phospho T680) antibody [EPR19958] - BSA and Azide free (ab251297)This data was developed using ab200061, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling BubR1 (phospho T680) with ab200061 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing positive staining on M phase HeLa cells, and LP treatment completely blocked the staining. (PMID:11792804). The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/250 dilution (red). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
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![Western blot - Anti-BubR1 (phospho T680) antibody [EPR19958] - BSA and Azide free (ab251297)](https://www.abcam.com/ps/products/251/ab251297/Images/ab251297-408171-anti-bubr1-phospho-t680-antibody-epr19958-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-BubR1 (phospho T680) antibody [EPR19958] - BSA and Azide free (ab251297)All lanes : Anti-BubR1 (phospho T680) antibody [EPR19958] (ab200061) at 1/2000 dilution
Lane 1 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated with 0.5 µM nocodazole for 24 hours
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated with 0.5 µM nocodazole for 24 hours, then treated with FastAP Thermosensitive Alkaline Phosphatase for 1 hour
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 120 kDa
Observed band size: 120 kDa
Exposure time: 3 minutesThis data was developed using ab200061, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Flow Cytometry - Anti-BubR1 (phospho T680) antibody [EPR19958] - BSA and Azide free (ab251297)](https://www.abcam.com/ps/products/251/ab251297/Images/ab251297-2-anti-bubr1-phospho-t680-antibody-epr19958-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-BubR1 (phospho T680) antibody [EPR19958] - BSA and Azide free (ab251297)This data was developed using ab200061, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, untreated or treated with 0.5 ng/ml nocodazole for 24 hours, labeling BubR1 (phospho T680) with ab200061 at 1/500 dilution compared with a Rabbit IgG,monoclonal [EPR25A]-Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. -
![Immunoprecipitation - Anti-BubR1 (phospho T680) antibody [EPR19958] - BSA and Azide free (ab251297)](https://www.abcam.com/ps/products/251/ab251297/Images/ab251297-1-anti-bubr1-phospho-t680-antibody-epr19958-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-BubR1 (phospho T680) antibody [EPR19958] - BSA and Azide free (ab251297)This data was developed using ab200061, the same antibody clone in a different buffer formulation.BubR1 (phospho T680) was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated with 0.5 μΜ nocodazole for 24 hours with ab200061 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab200061 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: HeLa whole cell lysate treated with 0.5 μΜ nocodazole for 24 hours 10µg (Input). Lane 2: ab200061 IP in HeLa whole cell lysate treated with 0.5 μΜ nocodazole for 24 hours. Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab200061 in HeLa whole cell lysate treated with 0.5 μΜ nocodazole for 24 hours. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 seconds. -
![Anti-BubR1 (phospho T680) antibody [EPR19958] - BSA and Azide free (ab251297)](https://www.abcam.com/ps/products/251/ab251297/Images/ab251297-5-benefits-of-recombinant-antibodies.png)
Anti-BubR1 (phospho T680) antibody [EPR19958] - BSA and Azide free (ab251297)
