Anti-UCP3+UCP1 antibody [EPR19006] - BSA and Azide free (ab251173)
![Anti-UCP3+UCP1 antibody [EPR19006] - BSA and Azide free (ab251173)](https://www.abcam.com/ps/products/251/ab251173/Images/ab251173-410300-anti-ucp3-ucp1-antibody-epr19006-bsa-and-azide-free-western-blot.jpg "Anti-UCP3+UCP1 antibody [EPR19006] - BSA and Azide free (ab251173)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19006] to UCP3+UCP1 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat
Overview
-
Product name
Anti-UCP3+UCP1 antibody [EPR19006] - BSA and Azide free
See all UCP3+UCP1 primary antibodies -
Description
Rabbit monoclonal [EPR19006] to UCP3+UCP1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
-
General notes
Ab251173 is the carrier-free version of ab193470. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251173 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR19006 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-UCP3+UCP1 antibody [EPR19006] - BSA and Azide free (ab251173)All lanes : Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470) at 1/1000 dilution
Lane 1 : Mouse UCP1 full length recombinant protein
Lane 2 : Mouse UCP2 full length recombinant protein
Lysates/proteins at 0.02 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 34, 33 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab193470, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Mouse UCP1 full length recombinant protein contains aa1-307 with a His-Tag®. Mouse UCP2 full length recombinant protein contains aa1-309 with a His-Tag®. These two full length recombinant proteins were made in-house.
-
![Western blot - Anti-UCP3+UCP1 antibody [EPR19006] - BSA and Azide free (ab251173)](https://www.abcam.com/ps/products/251/ab251173/Images/ab251173-407815-anti-ucp3-ucp1-antibody-epr19006-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-UCP3+UCP1 antibody [EPR19006] - BSA and Azide free (ab251173)Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470) at 1/1000 dilution + Mouse UCP3 full length recombinant protein at 0.02 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 34, 33 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondThis data was developed using ab193470, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Mouse UCP3 full length recombinant protein contains aa1-308 with a GST-Tag. This full length recombinant protein was made in-house.
-
![Western blot - Anti-UCP3+UCP1 antibody [EPR19006] - BSA and Azide free (ab251173)](https://www.abcam.com/ps/products/251/ab251173/Images/ab251173-405689-anti-ucp3-ucp1-antibody-epr19006-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-UCP3+UCP1 antibody [EPR19006] - BSA and Azide free (ab251173)All lanes : Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470) at 1/1000 dilution
Lane 1 : Mouse muscle tissue lysate
Lane 2 : Mouse lung tissue lysate
Lane 3 : C2C12 (Mouse myoblast cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 34, 33 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab193470, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 9080264).
-
![Western blot - Anti-UCP3+UCP1 antibody [EPR19006] - BSA and Azide free (ab251173)](https://www.abcam.com/ps/products/251/ab251173/Images/ab251173-403335-anti-ucp3-ucp1-antibody-epr19006-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-UCP3+UCP1 antibody [EPR19006] - BSA and Azide free (ab251173)All lanes : Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470) at 1/1000 dilution
Lane 1 : Mouse brown adipose lysate
Lane 2 : Rat brown adipose lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 34, 33 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?This data was developed using ab193470, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 1 minute; Lane 2: 15 seconds.
The expression profile observed is consistent with what has been described in the literature (PMID: 9080264).
-
![Immunoprecipitation - Anti-UCP3+UCP1 antibody [EPR19006] - BSA and Azide free (ab251173)](https://www.abcam.com/ps/products/251/ab251173/Images/ab251173-1-anti-ucp3-ucp1-antibody-epr19006-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-UCP3+UCP1 antibody [EPR19006] - BSA and Azide free (ab251173)This data was developed using ab193470, the same antibody clone in a different buffer formulation.UCP3 + UCP1 were immunoprecipitated from 1mg of Mouse skeletal muscle whole cell lysate with ab193470 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab193470 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution. Lane 1: Mouse skeletal muscle whole cell lysate, 10µg (Input). Lane 2: ab193470 IP in Mouse skeletal muscle whole cell lysate. Lane 3: Rabbit IgG, monoclonal - Isotype Control (ab172730) instead of ab193470 in Mouse skeletal muscle whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 1 second. -
![Anti-UCP3+UCP1 antibody [EPR19006] - BSA and Azide free (ab251173)](https://www.abcam.com/ps/products/251/ab251173/Images/ab251173-6-benefits-of-recombinant-antibodies.png)
Anti-UCP3+UCP1 antibody [EPR19006] - BSA and Azide free (ab251173)
