Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PGM1 antibody [EPR15241] (ab192876)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15241] to PGM1
  • Suitable for: ICC/IF, IP, WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PGM1 antibody [EPR15241]
    See all PGM1 primary antibodies

  • Description

    Rabbit monoclonal [EPR15241] to PGM1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2, 293T, Human fetal liver and fetal heart lysates; Mouse brain, heart, kidney, spleen, NIH 3T3 and RAW 264.7 lysates; Rat spleen, kidney, C6 and PC12 lysates; HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-PGM1 antibody [EPR15241] (ab192876)
    Western blot - Anti-PGM1 antibody [EPR15241] (ab192876)
    All lanes : Anti-PGM1 antibody [EPR15241] (ab192876) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Human fetal liver lysate
    Lane 4 : Human fetal heart lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 61 kDa
    Observed band size: 61 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-PGM1 antibody [EPR15241] (ab192876)
    Immunocytochemistry/ Immunofluorescence - Anti-PGM1 antibody [EPR15241] (ab192876)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa cells labeling PGM1 with ab192876 at 1/500 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary antibody at 1/400 dilution. Nuclear counter stain is DAPI (blue).

  • Flow Cytometry - Anti-PGM1 antibody [EPR15241] (ab192876)
    Flow Cytometry - Anti-PGM1 antibody [EPR15241] (ab192876)

    ab192876 staining PGM1 in A549 (human lung carcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. The sample was incubated with the primary antibody at a dilution of 1/90. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • Western blot - Anti-PGM1 antibody [EPR15241] (ab192876)
    Western blot - Anti-PGM1 antibody [EPR15241] (ab192876)
    All lanes : Anti-PGM1 antibody [EPR15241] (ab192876) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Mouse spleen lysate
    Lane 5 : Rat kidney lysate
    Lane 6 : Rat spleen lysate
    Lane 7 : C6 cell lysate
    Lane 8 : RAW 264.7 cell lysate
    Lane 9 : PC12 cell lysate
    Lane 10 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 61 kDa
    Observed band size: 61 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-PGM1 antibody [EPR15241] (ab192876)
    Immunoprecipitation - Anti-PGM1 antibody [EPR15241] (ab192876)

    Western blot analysis of PGM1 immunoprecipitated from Human fetal liver lysate with ab192876 at 1/60 dilution. Lane 1: Human fetal liver lysate. Lane 2: PBS instead of Human fetal liver lysate.
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-PGM1 antibody [EPR15241] (ab192876)
    Anti-PGM1 antibody [EPR15241] (ab192876)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-PGM1 antibody [EPR15241] (ab192876)

© 2021 Astana Biomed Group LLP. All rights reserved.