Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-PGM1 antibody [EPR15240] (ab188869)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15240] to PGM1
Suitable for: WB, Flow Cyt, IP, ICC/IF
Reacts with: Human

Product name

Anti-PGM1 antibody [EPR15240]
See all PGM1 primary antibodies
Description

Rabbit monoclonal [EPR15240] to PGM1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human fetal heart, fetal liver, HepG2, 293T and Jurkat lysates; 293T cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15240
Isotype

IgG
Research areas

Western blot - Anti-PGM1 antibody [EPR15240] (ab188869)

All lanes : Anti-PGM1 antibody [EPR15240] (ab188869) at 1/1000 dilution

Lane 1 : HepG2 cell lysate
Lane 2 : Jurkat cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 61 kDa
Immunoprecipitation - Anti-PGM1 antibody [EPR15240] (ab188869)

Western blot analysis of immunoprecipitation pellet from Human fetal liver lysate immunoprecipitated using ab188869 at 1/50 dilution.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Flow Cytometry - Anti-PGM1 antibody [EPR15240] (ab188869)

Flow cytometric analysis of 293T cells (paraformaldehyde-fixed, 2%) labeling PGM1 with ab188869 at 1/360 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-PGM1 antibody [EPR15240] (ab188869)

Immunofluorescent analysis of 293T cells (4% Paraformaldehyde-fixed) labeling PGM1 with ab188869 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Western blot - Anti-PGM1 antibody [EPR15240] (ab188869)

All lanes : Anti-PGM1 antibody [EPR15240] (ab188869) at 1/1000 dilution

Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal liver lysate
Lane 3 : 293T lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/500 dilution

Predicted band size: 61 kDa
Anti-PGM1 antibody [EPR15240] (ab188869)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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