Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [OTI1C4] to GALE
Suitable for: IHC-P, WB
Reacts with: Rat, Dog, Human, African green monkey
Isotype: IgG1

Product name

Anti-GALE antibody [OTI1C4]
See all GALE primary antibodies
Description

Mouse monoclonal [OTI1C4] to GALE
Host species

Mouse
Tested applications

Suitable for: IHC-P, WBmore details
Species reactivity

Reacts with: Rat, Dog, Human, African green monkey
Immunogen

Recombinant full length protein corresponding to Human GALE aa 1 to the C-terminus. Produced in HEK-293T cells (NP_000394).
Database link: Q14376

Run BLAST with Run BLAST with
Positive control
- WB: HEK-293T cell lysate transfected with the pCMV6-ENTRY GALE cDNA; HepG2, HeLa, HT-29, A549, COS-7, Jurkat, MDCK, PC-12 and MCF7 cell extracts; Human testis, omentum, uterus, breast, brain, liver, ovary, thyroid gland, colon and spleen extracts. IHC-P: Human breast adenocarcinoma, colon adenocarcinoma, kidney, liver, ovary adenocarcinoma, prostate, prostate carcinoma, bladder carcinoma and lymph node tissues.
General notes

The clone number has been updated from 1C4 to OTI1C4, both clone numbers name the same clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

OTI1C4
Isotype

IgG1
Research areas

Western blot - Anti-GALE antibody [OTI1C4] (ab118033)

All lanes : Anti-GALE antibody [OTI1C4] (ab118033) at 1/500 dilution

Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY GALE cDNA

Lysates/proteins at 5 µg per lane.

Predicted band size: 38 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)

Paraffin-embedded human bladder carcinoma tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)

Paraffin-embedded human lymph node tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.
Western blot - Anti-GALE antibody [OTI1C4] (ab118033)

All lanes : Anti-GALE antibody [OTI1C4] (ab118033) at 1/500 dilution

Lane 1 : Human testis extract
Lane 2 : Human omentum extract
Lane 3 : Human uterus extract
Lane 4 : Human breast extract
Lane 5 : Human brain extract
Lane 6 : Human liver extract
Lane 7 : Human ovary extract
Lane 8 : Human thyroid gland extract
Lane 9 : Human colon extract
Lane 10 : Human spleen extract

Lysates/proteins at 10 µg per lane.

Predicted band size: 38 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)

Paraffin-embedded human prostate carcinoma tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)

Paraffin-embedded human prostate tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)

Paraffin-embedded human ovary adenocarcinoma tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)

Paraffin-embedded human liver tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)

Paraffin-embedded human kidney tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)

Paraffin-embedded human colon adenocarcinoma tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)

Paraffin-embedded human breast adenocarcinoma tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.
Western blot - Anti-GALE antibody [OTI1C4] (ab118033)

All lanes : Anti-GALE antibody [OTI1C4] (ab118033) at 1/500 dilution

Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 3 : HT-29 (Human colorectal adenocarcinoma cell line) cell extract
Lane 4 : A549 (human lung carcinoma cell line) cell extract
Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) cell extract
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
Lane 7 : MDCK (Canine kidney cell line) cell extract
Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell extract
Lane 9 : MCF7 (Human breast adenocarcinoma cell line) cell extract

Lysates/proteins at 35 µg per lane.

Predicted band size: 38 kDa

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