Anti-GALE antibody [OTI1C4] (ab118033)
![Anti-GALE antibody [OTI1C4] (ab118033)](https://www.abcam.com/ps/products/118/ab118033/Images/ab118033-130015-anti-gale-antibody-oti1c4-western-blot.png "Anti-GALE antibody [OTI1C4] (ab118033)")
Key features and details
- Mouse monoclonal [OTI1C4] to GALE
- Suitable for: IHC-P, WB
- Reacts with: Rat, Dog, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-GALE antibody [OTI1C4]
See all GALE primary antibodies -
Description
Mouse monoclonal [OTI1C4] to GALE -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Dog, Human, African green monkey -
Immunogen
Recombinant full length protein corresponding to Human GALE aa 1 to the C-terminus. Produced in HEK-293T cells (NP_000394).
Database link: Q14376 -
Positive control
- WB: HEK-293T cell lysate transfected with the pCMV6-ENTRY GALE cDNA; HepG2, HeLa, HT-29, A549, COS-7, Jurkat, MDCK, PC-12 and MCF7 cell extracts; Human testis, omentum, uterus, breast, brain, liver, ovary, thyroid gland, colon and spleen extracts. IHC-P: Human breast adenocarcinoma, colon adenocarcinoma, kidney, liver, ovary adenocarcinoma, prostate, prostate carcinoma, bladder carcinoma and lymph node tissues.
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General notes
The clone number has been updated from 1C4 to OTI1C4, both clone numbers name the same clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
OTI1C4 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-GALE antibody [OTI1C4] (ab118033)All lanes : Anti-GALE antibody [OTI1C4] (ab118033) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY GALE cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 38 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)](https://www.abcam.com/ps/products/118/ab118033/Images/ab118033-314129-anti-gale-antibody-oti1c4-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)Paraffin-embedded human bladder carcinoma tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)](https://www.abcam.com/ps/products/118/ab118033/Images/ab118033-314130-anti-gale-antibody-oti1c4-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)Paraffin-embedded human lymph node tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.
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![Western blot - Anti-GALE antibody [OTI1C4] (ab118033)](https://www.abcam.com/ps/products/118/ab118033/Images/ab118033-314121-anti-gale-antibody-oti1c4-western-blot.jpg)
Western blot - Anti-GALE antibody [OTI1C4] (ab118033)All lanes : Anti-GALE antibody [OTI1C4] (ab118033) at 1/500 dilution
Lane 1 : Human testis extract
Lane 2 : Human omentum extract
Lane 3 : Human uterus extract
Lane 4 : Human breast extract
Lane 5 : Human brain extract
Lane 6 : Human liver extract
Lane 7 : Human ovary extract
Lane 8 : Human thyroid gland extract
Lane 9 : Human colon extract
Lane 10 : Human spleen extract
Lysates/proteins at 10 µg per lane.
Predicted band size: 38 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)](https://www.abcam.com/ps/products/118/ab118033/Images/ab118033-314128-anti-gale-antibody-oti1c4-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)Paraffin-embedded human prostate carcinoma tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)](https://www.abcam.com/ps/products/118/ab118033/Images/ab118033-314127-anti-gale-antibody-oti1c4-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)Paraffin-embedded human prostate tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)](https://www.abcam.com/ps/products/118/ab118033/Images/ab118033-314126-anti-gale-antibody-oti1c4-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)Paraffin-embedded human ovary adenocarcinoma tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)](https://www.abcam.com/ps/products/118/ab118033/Images/ab118033-314125-0335.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)Paraffin-embedded human liver tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)](https://www.abcam.com/ps/products/118/ab118033/Images/ab118033-314124-anti-gale-antibody-oti1c4-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)Paraffin-embedded human kidney tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)](https://www.abcam.com/ps/products/118/ab118033/Images/ab118033-314123-anti-gale-antibody-oti1c4-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)Paraffin-embedded human colon adenocarcinoma tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)](https://www.abcam.com/ps/products/118/ab118033/Images/ab118033-314122-anti-gale-antibody-oti1c4-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)Paraffin-embedded human breast adenocarcinoma tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.
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![Western blot - Anti-GALE antibody [OTI1C4] (ab118033)](https://www.abcam.com/ps/products/118/ab118033/Images/ab118033-130014-anti-gale-antibody-oti1c4-western-blot.jpg)
Western blot - Anti-GALE antibody [OTI1C4] (ab118033)All lanes : Anti-GALE antibody [OTI1C4] (ab118033) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 3 : HT-29 (Human colorectal adenocarcinoma cell line) cell extract
Lane 4 : A549 (human lung carcinoma cell line) cell extract
Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) cell extract
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
Lane 7 : MDCK (Canine kidney cell line) cell extract
Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell extract
Lane 9 : MCF7 (Human breast adenocarcinoma cell line) cell extract
Lysates/proteins at 35 µg per lane.
Predicted band size: 38 kDa
