Anti-G3BP (phospho S232) antibody (ab131243)
Key features and details
- Rabbit polyclonal to G3BP (phospho S232)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-G3BP (phospho S232) antibody
See all G3BP primary antibodies -
Description
Rabbit polyclonal to G3BP (phospho S232) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic phosphopeptide conjugated to KLH, derived from around phosphorylation site of serine 232 (S-S-S(p)-P-A) of Human G3BP (UniProt Q13283).
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Positive control
- Hela cells; Extracts from 293 cells treated with starvation; Human breast carcinoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab131243 was purified by affinity chromatography using peptide-specific phosphopeptide. Non-phosphosepcific antibodies were removed by chromatography using non-specific peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunofluorescence analysis of methanol-fixed Hela cells labelling G3BP (phospho S232) with ab131243 at 1/100 dilution.
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All lanes : Anti-G3BP (phospho S232) antibody (ab131243) at 1/500 dilution
Lane 1 : Untreated 293 cell extract
Lane 2 : Extract from 293 cells treated with starvation
Predicted band size: 52 kDa
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Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling G3BP (phospho S232) with ab131243 at 1/50 dilution. Right panel was preincubated with blocking peptide.