Anti-HTF9C/TRMT2A antibody [1G8] (ab230922)
![Anti-HTF9C/TRMT2A antibody [1G8] (ab230922)](https://www.abcam.com/ps/products/230/ab230922/Images/ab230922-393629-anti-htf9ctrmt2a-antibody-1g8-western-blot-human-knockout-lysate.jpg "Anti-HTF9C/TRMT2A antibody [1G8] (ab230922)")
Key features and details
- Mouse monoclonal [1G8] to HTF9C/TRMT2A
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-HTF9C/TRMT2A antibody [1G8]
See all HTF9C/TRMT2A primary antibodies -
Description
Mouse monoclonal [1G8] to HTF9C/TRMT2A -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human HTF9C/TRMT2A. (NP_073564). Produced in HEK293T cells.
Database link: Q8IZ69 -
Positive control
- WB: HeLa, MOLT-4, pCMV6-ENTRY HTF9C/TRMT2A-transfected HEK-293T whole cell lysates. IHC-P: Human testis tissue.
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General notes
This product was previously labelled as HTF9C
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
1G8 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-HTF9C/TRMT2A antibody [1G8] (ab230922)All lanes : Anti-HTF9C/TRMT2A antibody [1G8] (ab230922) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TRMT2A knockout HeLa cell lysate
Lane 3 : MOLT-4 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 69 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab230922 observed at 75 kDa. Red - loading control ab181602 observed at 36 kDa.
ab230922 Anti-HTF9C/TRMT2A antibody [1G8] was shown to specifically react with HTF9C/TRMT2A in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265160 (knockout cell lysate ab258253) was used. Wild-type and HTF9C/TRMT2A knockout samples were subjected to SDS-PAGE. ab230922 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated at room temperature for 2. 5 hours at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HTF9C/TRMT2A antibody [1G8] (ab230922)](https://www.abcam.com/ps/products/230/ab230922/Images/ab230922-300644-HTF9CIHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HTF9C/TRMT2A antibody [1G8] (ab230922)Formalin-fixed, paraffin-embedded human testis tissue stained for HTF9C/TRMT2A using ab230922 at 10 μg/ml in immunohistochemical analysis.
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![Western blot - Anti-HTF9C/TRMT2A antibody [1G8] (ab230922)](https://www.abcam.com/ps/products/230/ab230922/Images/ab230922-300647-HTF9CWB.jpg)
Western blot - Anti-HTF9C/TRMT2A antibody [1G8] (ab230922)All lanes : Anti-HTF9C/TRMT2A antibody [1G8] (ab230922) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : pCMV6-ENTRY HTF9C/TRMT2A-transfected HEK-293T whole cell lysate
Lysates/proteins at 5 µg per lane.
Developed using the ECL technique.
Predicted band size: 69 kDa
