Anti-HTF9C/TRMT2A antibody - C-terminal (ab229733)
Key features and details
- Rabbit polyclonal to HTF9C/TRMT2A - C-terminal
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HTF9C/TRMT2A antibody - C-terminal
See all HTF9C/TRMT2A primary antibodies -
Description
Rabbit polyclonal to HTF9C/TRMT2A - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human HTF9C/TRMT2A (C terminal). The exact sequence is proprietary.
Database link: Q8IZ69 -
Positive control
- WB: HEK-293T, A431 and HeLa whole cell extracts. IHC-P: Human lung cancer tissue. ICC/IF: HeLa cells.
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General notes
This product was previously labelled as HTF9C
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HTF9C/TRMT2A antibody - C-terminal (ab229733)All lanes : Anti-HTF9C/TRMT2A antibody - C-terminal (ab229733) at 1/3500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 69 kDa7.5% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HTF9C/TRMT2A antibody - C-terminal (ab229733)Paraffin-embedded human lung cancer tissue stained for HTF9C/TRMT2A using ab229733 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-HTF9C/TRMT2A antibody - C-terminal (ab229733)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HTF9C/TRMT2A (green) using ab229733 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
