Mouse PDK4 ELISA Kit (ab215544)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 45 pg/ml
- Range: 0.234 ng/ml - 15 ng/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse PDK4 ELISA Kit
See all PDK4 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5 2% Inter-assay Sample n Mean SD CV% Overall 3 9% -
Sample type
Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
45 pg/ml -
Range
0.234 ng/ml - 15 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 98 94% - 103% Mouse muscle extract 98 95% - 100% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow -
Product overview
Mouse PDK4 ELISA Kit (ab215544) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PDK4 protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse PDK4 with 45 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
PDK4 is a mitochondrial kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. PDH phosphorylation inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation. PDK4 plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, PDK4 plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, PDK4 mediates cellular responses to glucose levels and to a high-fat diet. PDK4 regulates both fatty acid oxidation and de novo fatty acid biosynthesis. PDK4 plays a role in the generation of reactive oxygen species.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse PDK4 Capture Antibody 1 x 600µl 10X Mouse PDK4 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Mouse PDK4 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism. -
Tissue specificity
Ubiquitous; highest levels of expression in heart and skeletal muscle. -
Sequence similarities
Belongs to the PDK/BCKDK protein kinase family.
Contains 1 histidine kinase domain. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Alternative names
- [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4
- FLJ40832
- mitochondrial
see all -
Database links
- Entrez Gene: 27273 Mouse
- SwissProt: O70571 Mouse
- Unigene: 235547 Mouse
Images
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Other - Mouse PDK4 ELISA Kit (ab215544)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mosue PDK4 standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native PDK4 in C2C12 cell extract sample, mouse skeletal muscle tissue extract and mouse and rat heart tissue extracts.Interpolated concentrations of native PDK4 in C2C12 cell extract sample based on a 500 μg/mL extract load, mouse skeletal muscle tissue extract based on a 500 μg/mL extract load, and mouse and rat heart tissue extract based on a 300 μg/mL extract load. The concentrations of PDK4 were measured in duplicate and interpolated from the PDK4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PDK4 concentration was determined to be 4.8 ng/mL in C2C12 extract, 8.4 ng/mL in mouse skeletal muscle extract, 7.8 ng/mL in mouse heart extract, and 10.5 ng/mL in rat heart extract.
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Interpolated concentrations of PDK4 in various cell and tissue extracts.Interpolated concentrations of PDK4 in various cell and tissue extracts. The concentrations of PDK4 were measured in three different dilutions in duplicate and interpolated from the PDK4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in ng PDK4 per mg of extract (mean +/- SD, n=3). PDK4 concentration was determined to be 9.9 ng/mg in C2C12 cell extract, 17.3 ng/mg in mouse skeletal muscle extract, 26.7 ng/mg in mouse heart, and 36.7 ng/mg in rat heart.
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Interpolated concentrations of PDK4 in various species heart tissue extracts.Other species reactivity was determined by measuring a 300 µg/mL extract load of various species heart tissue extract, and interpolating the protein concentrations from the mouse standard curve.
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Sandwich ELISA - Mouse PDK4 ELISA Kit (ab215544)To learn more about the advantages of recombinant antibodies see here.
