Recombinant rat C5 protein (Active) (ab255801)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: PBS, 5% Trehalose
0.22 µm filteredThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
Images
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SDS-PAGE - Rat C5 protein (Active) (ab255801)
SDS-PAGE - Rat C5 protein (Active) (ab255801) under reducing (R) condition.
The gel was stained overnight with Coomassie Blue.
The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 76.1 kDa (β chain) and 114.8 kDa (α chain). The protein migrates as 70 kDa (β chain) and 110 kDa (α chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.
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Functional Studies - Rat C5 protein (Active) (ab255801)Immobilized ab255801 at 2 μg/mL (100 μL/well) can bind anti-C5a (Human IgG1) with a linear range of 0.4-12.5 ng/mL.
