Anti-PGM1 antibody (ab232959)
Key features and details
- Rabbit polyclonal to PGM1
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
Overview
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Product name
Anti-PGM1 antibody
See all PGM1 primary antibodies -
Description
Rabbit polyclonal to PGM1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Mouse, Rat, Rabbit, Cow, Cynomolgus monkey
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Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human PGM1 aa 1-250. Expressed in E.coli. N-terminal tags.
Database link: P36871 -
Positive control
- WB: Recombinant human PGM1 protein; HeLa and HEK-293T cell lysates; Pig liver lysate. IHC-P: Human cerebrum, liver, kidney, stomach, breast cancer and prostate tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Research areas
Images
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Western blot - Anti-PGM1 antibody (ab232959)All lanes : Anti-PGM1 antibody (ab232959) at 1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lanes 3-4 : Pig liver lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 61 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM1 antibody (ab232959)Formalin-fixed, paraffin-embedded human kidney tissue stained for PGM1 using ab232959 at 10 μg/ml in immunohistochemical analysis.
Secomdary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM1 antibody (ab232959)Formalin-fixed, paraffin-embedded human cerebrum tissue stained for PGM1 using ab232959 at 10 μg/ml in immunohistochemical analysis.
Secomdary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM1 antibody (ab232959)Formalin-fixed, paraffin-embedded human liver tissue stained for PGM1 using ab232959 at 10 μg/ml in immunohistochemical analysis.
Secomdary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM1 antibody (ab232959)Formalin-fixed, paraffin-embedded human stomach tissue stained for PGM1 using ab232959 at 10 μg/ml in immunohistochemical analysis.
Secomdary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM1 antibody (ab232959)Formalin-fixed, paraffin-embedded human breast cancer tissue stained for PGM1 using ab232959 at 10 μg/ml in immunohistochemical analysis.
Secomdary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM1 antibody (ab232959)Formalin-fixed, paraffin-embedded human prostate tissue stained for PGM1 using ab232959 at 10 μg/ml in immunohistochemical analysis.
Secomdary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Western blot - Anti-PGM1 antibody (ab232959)Anti-PGM1 antibody (ab232959) at 1 µg/ml + Recombinant human PGM1 protein
Secondary
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 61 kDa
