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Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10734(B)] to PFKM - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free
    See all PFKM primary antibodies

  • Description

    Rabbit monoclonal [EPR10734(B)] to PFKM - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: SH-SY5Y cells.

  • General notes

    ab232495 is the carrier-free version of ab154804 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab232495 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Fructose 6 Phosphate Kinase

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)
    Flow Cytometry - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PFKM with purified ab154804 at 1:300 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154804).

  • Immunocytochemistry/ Immunofluorescence - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)
    Immunocytochemistry/ Immunofluorescence - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)

    Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling PFKM with Purified ab154804 at 1:300 dilution (8.7μg/ml). Cells were fixed in 100% Methanol and cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154804).

  • Immunocytochemistry/ Immunofluorescence - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)
    Immunocytochemistry/ Immunofluorescence - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)

    Immunofluorescence analysis of HeLa cells labeling PFKM with ab154804 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154804).

  • Flow Cytometry - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)
    Flow Cytometry - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)

    Flow cytometric analysis of permeabilized SHSY5Y cells labeling PFKM with ab154804 at 1/100 dilution (red) or a rabbit IgG (green - negative control).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154804).

  • Immunocytochemistry/ Immunofluorescence - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)
    Immunocytochemistry/ Immunofluorescence - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)

    Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (human neuroblastoma epithelial cell) cells labeling PFKM with Purified ab154804 at 1:300 dilution (8.7μg/ml). Cells were fixed in 100% Methanol and cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154804).

  • Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)
    Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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