Antibodies, Proteins, Kits and Reagents for Life Science

318 288 ₸ Product size

100 µl 318 288 ₸

Order now and get it on Tuesday March 02, 2021

Anti-PFKM antibody [EPR10734(B)] (ab154804)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10734(B)] to PFKM
Suitable for: WB, ICC/IF, Flow Cyt
Reacts with: Mouse, Rat, Human, Pig

Product name

Anti-PFKM antibody [EPR10734(B)]
See all PFKM primary antibodies
Description

Rabbit monoclonal [EPR10734(B)] to PFKM
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Rat Human Pig

See all applications and species data

Immunogen

Synthetic peptide within Human PFKM. The exact sequence is proprietary.
(Peptide available as ab207505)
Positive control
- Fetal brain, fetal heart, Ramos, SHSY5Y and HeLa lysates; HeLa and SHSY5Y cells; Rat skeletal; Mouse skeletal; Pig heart lysates.
General notes
This product was previously labelled as Fructose 6 Phosphate Kinase

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR10734(B)
Isotype

IgG
Research areas

Western blot - Anti-PFKM antibody [EPR10734(B)] (ab154804)

Anti-PFKM antibody [EPR10734(B)] (ab154804) at 1/10000 dilution (purified) + Pig heart lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 85 kDa

Blocking and diluting buffer : 5% NFDM/TBST
Flow Cytometry - Anti-PFKM antibody [EPR10734(B)] (ab154804)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PFKM with purified ab154804 at 1:300 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunocytochemistry/ Immunofluorescence - Anti-PFKM antibody [EPR10734(B)] (ab154804)

Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling PFKM with Purified ab154804 at 1:300 dilution (8.7μg/ml). Cells were fixed in 100% Methanol and cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Western blot - Anti-PFKM antibody [EPR10734(B)] (ab154804)

All lanes : Anti-PFKM antibody [EPR10734(B)] (ab154804) at 1/2000 dilution (purified)

Lane 1 : Rat skeletal muscle lysates
Lane 2 : Mouse skeletal muscle lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 85 kDa

Blocking and diluting buffer : 5% NFDM/TBST
Western blot - Anti-PFKM antibody [EPR10734(B)] (ab154804)

Anti-PFKM antibody [EPR10734(B)] (ab154804) at 1/2000 dilution (purified) + Human fetal heart lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 85 kDa

Blocking and diluting buffer : 5% NFDM/TBST
Western blot - Anti-PFKM antibody [EPR10734(B)] (ab154804) This image is courtesy of an Abreview submitted by Eric England

Western blot analysis of Pig skeletal muscle tissue lysate using ab154804 (1:2000 in 1x PBS + 0.1% Tween-20 for 1 hour at 22°C) to label PFKM. A IRDye® 680-conjugated Donkey anti-rabbit IgG (1:40000) was used as the secondary antibody. The image shows increasing amounts of loaded protein for antibody optimization. The far left lane is the molecular weight standard.
See Abreview
Western blot - Anti-PFKM antibody [EPR10734(B)] (ab154804)

All lanes : Anti-PFKM antibody [EPR10734(B)] (ab154804) at 1/1000 dilution

Lane 1 : Fetal brain lysate
Lane 2 : Fetal heart lysate
Lane 3 : Ramos lysate
Lane 4 : SHSY5Y lysate
Lane 5 : HeLa lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 85 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PFKM antibody [EPR10734(B)] (ab154804)

Immunofluorescence analysis of HeLa cells labeling PFKM with ab154804 at 1/50 dilution.
Flow Cytometry - Anti-PFKM antibody [EPR10734(B)] (ab154804)

Flow cytometric analysis of permeabilized SHSY5Y cells labeling PFKM with ab154804 at 1/100 dilution (red) or a rabbit IgG (green - negative control).
Anti-PFKM antibody [EPR10734(B)] (ab154804)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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