Anti-NCF1/p47-phox (phospho S359) antibody (ab74095)
Key features and details
- Rabbit polyclonal to NCF1/p47-phox (phospho S359)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NCF1/p47-phox (phospho S359) antibody
See all NCF1/p47-phox primary antibodies -
Description
Rabbit polyclonal to NCF1/p47-phox (phospho S359) -
Host species
Rabbit -
Specificity
ab74095 detects endogenous levels of NCF1/p47-phox only when phosphorylated at serine 359.
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Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human NCF1/p47-phox (phospho S359).
Sequence:Q-R-Sp-K-P
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Positive control
- Human colon carcinoma tissue, HeLa cells and HeLa cell extracts treated with nocodazole (1ug/ml, 18hours) or TSA (400nM, 24hours).
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General notes
Previously labelled as NCF1.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab74095 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NCF1/p47-phox (phospho S359) antibody (ab74095)All lanes : Anti-NCF1/p47-phox (phospho S359) antibody (ab74095) at 1/500 dilution
Lane 1 : HeLa cell extracts treated
with nocodazole (1ug/ml, 18hours)
Lane 2 : HeLa cell extracts treated
with TSA (400nM, 24hours)
Lane 3 : HeLa cell extracts treated
with nocodazole (1ug/ml, 18hours) and with TSA (400nM, 24hours) with the immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 45 kDa
Observed band size: 45 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCF1/p47-phox (phospho S359) antibody (ab74095)ab74095 at 1/50 dilution staining NCF1/p47-phox in human colon carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
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Immunocytochemistry/ Immunofluorescence - Anti-NCF1/p47-phox (phospho S359) antibody (ab74095)ab74095 at 1/500 dilution staining NCF1/p47-phox in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
