Anti-NCF1/p47-phox antibody (ab795)
Key features and details
- Goat polyclonal to NCF1/p47-phox
- Suitable for: WB
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-NCF1/p47-phox antibody
See all NCF1/p47-phox primary antibodies -
Description
Goat polyclonal to NCF1/p47-phox -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt PigWB MouseHuman
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Immunogen
Synthetic peptide corresponding to Human NCF1/p47-phox aa 378-390 (C terminal).
Sequence:CSESTKRKLASAV
(Peptide available asab22781) -
Positive control
- WB: U937, Daudi, and U251 cell lysates, Mouse thymus and pig spleen tissue lysates.
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General notes
Principal Names - NCF1 / p47-phox; NCF1; p47phox; Neutrophil cytosolic factor-1 (47 kDa, chronic granulomatous disease, autosomal 1) Official Gene Symbol - NCF1 GenBank Accession Number – NP_000256 LocusLink ID - 4687
NCF1 / p47-phox is a cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils.
Previously labelled as NCF1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Primary antibody notes
NCF1 is a cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NCF1/p47-phox antibody (ab795)Lane 1 : Anti-NCF1/p47-phox antibody (ab795) at 0.2 µg/ml
Lane 2 : Anti-NCF1/p47-phox antibody (ab795) at 0.01 µg/ml
Lanes 3-4 : Anti-NCF1/p47-phox antibody (ab795) at 0.3 µg/ml
Lane 1 : U937 (human histiocytic lymphoma cell line) cell lysate
Lane 2 : Daudi (human Burkitt's lymphoma cell line) cell lysate
Lane 3 : U-251 MG (formally U-373 MG) (human brain glioma cell line) whole cell lysate
Lane 4 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 45 kDaLysates prepared in RIPA buffer. Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.
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Western blot - Anti-NCF1/p47-phox antibody (ab795)All lanes : Anti-NCF1/p47-phox antibody (ab795) at 1 µg/ml
Lane 1 : Mouse thymus lysate
Lane 2 : Pig spleen lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 45 kDaLysates prepared in RIPA buffer. Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.
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Western blot - Anti-NCF1/p47-phox antibody (ab795)Anti-NCF1/p47-phox antibody (ab795) at 1.5 µg/ml +Recombinant Human NCF1/p47-phox protein (ab98054) at 0.1 µg
Secondary
Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Exposure time: 8 minutes
