Anti-PDK1 antibody [EPR13015] - C-terminal (ab180171)
![Anti-PDK1 antibody [EPR13015] - C-terminal (ab180171)](https://www.abcam.com/ps/products/180/ab180171/Images/ab180171-259076-ab180171KOWB.jpg "Anti-PDK1 antibody [EPR13015] - C-terminal (ab180171)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13015] to PDK1 - C-terminal
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-PDK1 antibody [EPR13015] - C-terminal
See all PDK1 primary antibodies -
Description
Rabbit monoclonal [EPR13015] to PDK1 - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human PDK1 aa 400 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: Q15118 -
Positive control
- Fetal heart, LNCaP and HepG2 lysates.
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General notes
This product was previously labelled as Mitochondrial Pyruvate dehydrogenase kinase 1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13015 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PDK1 antibody [EPR13015] - C-terminal (ab180171)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Mitochondrial Pyruvate dehydrogenase kinase 1 knockout HAP1 cell lysate (20 µg)
Lane 3: Human fetal heart tissue lysate (20 µg)
Lane 4: LnCaP cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab180171 observed at 46 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab180171 was shown to specifically react with Mitochondrial Pyruvate dehydrogenase kinase 1 in wild-type HAP1 cells along with additional cross reactive bands. No bands were observed when Mitochondrial Pyruvate dehydrogenase kinase 1 knockout samples were examined. Wild-type and Mitochondrial Pyruvate dehydrogenase kinase 1 knockout samples were subjected to SDS-PAGE. ab180171 and ab8245 (loading control to GAPDH) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-PDK1 antibody [EPR13015] - C-terminal (ab180171)](https://www.abcam.com/ps/products/180/ab180171/Images/ab180171-208765-ab180171wb.jpg)
Western blot - Anti-PDK1 antibody [EPR13015] - C-terminal (ab180171)All lanes : Anti-PDK1 antibody [EPR13015] - C-terminal (ab180171) at 1/10000 dilution
Lane 1 : Fetal heart lysate
Lane 2 : LNCaP cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 49 kDa
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![Anti-PDK1 antibody [EPR13015] - C-terminal (ab180171)](https://www.abcam.com/ps/products/180/ab180171/Images/ab180171-3-benefits-of-recombinant-antibodies.png)
Anti-PDK1 antibody [EPR13015] - C-terminal (ab180171)
