Antibodies, Proteins, Kits and Reagents for Life Science

301 536 ₸ Product size

100 µl 301 536 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Mouse monoclonal [4A11] to PDK1
Suitable for: WB, ICC/IF, IHC-P, Flow Cyt
Reacts with: Mouse, Rat, Human, African green monkey
Isotype: IgG1

Product name

Anti-PDK1 antibody [4A11]
See all PDK1 primary antibodies
Description

Mouse monoclonal [4A11] to PDK1
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Mouse Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human PDK1 aa 308-436.
Database link: Q15118
Positive control
- Hela, Jurkat, HepG2, PC-12, and Cos7 cell lysate, human breast cancer and Human brain tissues, HELA and Lovo cells
General notes

This product was previously labelled as Mitochondrial Pyruvate dehydrogenase kinase 1

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: PBS
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from tissue culture supernatant.
Clonality

Monoclonal
Clone number

4A11
Isotype

IgG1
Research areas

Western blot - Anti-PDK1 antibody [4A11] (ab110025)

Lanes 1-2 : Anti-PDK1 antibody [4A11] (ab110025) at 1/500 dilution
Lane 3 : Beta-actin antibody at 1/10000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : NIH3T3 cell lysate

Lysates/proteins at 15 µl per lane.

Secondary
All lanes : HRP-Goat Anti-Mouse IgG (Fc) at 1/10000 dilution

Predicted band size: 49 kDa

12% SDS gel.

Block the membrane for 1 h at room temperature or overnight at 4°C using blocking buffer, 5% milk.

Incubate in ab110025 overnight at 4°C.

Incubation at room temperature for 1 hour with the secondary antibody.
Western blot - Anti-PDK1 antibody [4A11] (ab110025)

All lanes : Anti-PDK1 antibody [4A11] (ab110025) at 1/500 dilution

Lane 1 : NIH3T3 cell lysate
Lane 2 : Hela cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : PC-12 cell lysate
Lane 6 : Cos7 cell lysate

Predicted band size: 49 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK1 antibody [4A11] (ab110025)

Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using ab110025 aty a dilution of 1/200 with DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK1 antibody [4A11] (ab110025)

Immunohistochemical analysis of paraffin-embedded Human brain tissues using ab110025 aty a dilution of 1/200 with DAB staining.
Immunocytochemistry/ Immunofluorescence - Anti-PDK1 antibody [4A11] (ab110025)

Immunofluorescence analysis of HELA cells using ab110025 at a dilution of 1/200 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry - Anti-PDK1 antibody [4A11] (ab110025)

Flow cytometric analysis of Lovo cells using ab110025 (green) and negative control (purple).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com