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Developmental Biology Lineage specification Ectoderm

Anti-5T4 antibody [B3F1] (ab254168)

Price and availability

321 638 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-5T4 antibody [B3F1] (ab254168)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [B3F1] to 5T4
  • Suitable for: Flow Cyt
  • Reacts with: Mouse

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Overview

  • Product name

    Anti-5T4 antibody [B3F1]
    See all 5T4 primary antibodies
  • Description

    Mouse monoclonal [B3F1] to 5T4
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    See all applications and species data
  • Immunogen

    Recombinant fragment within Mouse 5T4. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q9Z0L0

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Flow Cyt: NIH/3T3 cells.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    B3F1
  • Isotype

    IgG2a
  • Research areas

    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Stem Cells
    • Lineage Markers
    • Endoderm
    • Stem Cells
    • Lineage Markers
    • Mesoderm
    • Cancer
    • Tumor immunology
    • Tumor-associated antigens
    • Developmental Biology
    • Lineage specification
    • Endoderm
    • Developmental Biology
    • Lineage specification
    • Ectoderm
    • Developmental Biology
    • Lineage specification
    • Mesoderm

Images

  • Flow Cytometry - Anti-5T4 antibody [B3F1] (ab254168)
    Flow Cytometry - Anti-5T4 antibody [B3F1] (ab254168)

    Flow cytometric analysis of RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage, Left) / NIH/3T3 (Mouse embryonic fibroblast, Right) labeling 5T4 with ab254168 at 1.012µg/ml (red) compared with Mouse monoclonal IgG Isotype Control (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Mouse IgG (Alexa Fluor® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody.

    Negative control: RAW 264.7 (PMID: 10366710).

    Gated on viable cells.

  • Anti-5T4 antibody [B3F1] (ab254168)
    Anti-5T4 antibody [B3F1] (ab254168)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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