Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-5T4 antibody [EPR5529] (ab134162)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5529] to 5T4
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-5T4 antibody [EPR5529]
    See all 5T4 primary antibodies

  • Description

    Rabbit monoclonal [EPR5529] to 5T4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human 5T4 aa 100-200 (extracellular). The exact sequence is proprietary.
    Database link: Q13641

  • Positive control

    • WB: HT-29, HT-1376, and Caco-2 cell lysates. IHC-P: Human placenta and endometrial cancer tissues.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5T4 antibody [EPR5529] (ab134162)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5T4 antibody [EPR5529] (ab134162)

    Immunohistochemical staining using ab134162 on paraffin embedded human placenta tissue.

  • Western blot - Anti-5T4 antibody [EPR5529] (ab134162)
    Western blot - Anti-5T4 antibody [EPR5529] (ab134162)
    Anti-5T4 antibody [EPR5529] (ab134162) at 1/500 dilution (unpurified) + HT-29 cell lysate at 20 µg

    Secondary
    Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 46 kDa
    Observed band size: 72-80 kDa
    why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-5T4 antibody [EPR5529] (ab134162)
    Western blot - Anti-5T4 antibody [EPR5529] (ab134162)
    Anti-5T4 antibody [EPR5529] (ab134162) at 1/5000 dilution (purified) + HT-29 cell lysate at 20 µg

    Secondary
    Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 46 kDa
    Observed band size: 72-80 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-5T4 antibody [EPR5529] (ab134162)
    Western blot - Anti-5T4 antibody [EPR5529] (ab134162)
    All lanes : Anti-5T4 antibody [EPR5529] (ab134162) at 1/1000 dilution (unpurified format)

    Lane 1 : HT-29 cell lysates
    Lane 2 : HT-1376 cell lysates
    Lane 3 : Caco-2 cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 46 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5T4 antibody [EPR5529] (ab134162)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5T4 antibody [EPR5529] (ab134162)

    Immunohistochemical staining using ab134162 on paraffin embedded human endometrial cancer.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5T4 antibody [EPR5529] (ab134162)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5T4 antibody [EPR5529] (ab134162)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling 5T4 with unpurified ab134162 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5T4 antibody [EPR5529] (ab134162)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5T4 antibody [EPR5529] (ab134162)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling 5T4 with purified ab134162 at 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • OI-RD Scanning - Anti-5T4 antibody [EPR5529] (ab134162)
    OI-RD Scanning - Anti-5T4 antibody [EPR5529] (ab134162)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-5T4 antibody [EPR5529] (ab134162)
    Anti-5T4 antibody [EPR5529] (ab134162)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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