Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human Cripto ELISA Kit (ab245719)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 9.6 pg/ml
  • Range: 26.56 pg/ml - 1700 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Cripto ELISA Kit
    See all Cripto1/CRIPTO kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    protein 8 1.7%

  • Sample type

    Cell culture supernatant, Serum, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    9.6 pg/ml

  • Range

    26.56 pg/ml - 1700 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95 % - %
    Serum 86 % - %
    EDTA Plasma 86 % - %
    Cit plasma 86 % - %

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human Cripto ELISA Kit (ab245719) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cripto protein in cell culture supernatant, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Cripto with 9.6 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human Cripto Capture Antibody 1 x 600µl
    10X Human Cripto Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BI 1 x 6ml
    Human Cripto Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.

  • Tissue specificity

    Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts.

  • Sequence similarities

    Contains 1 EGF-like domain.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Alternative names

    • CR
    • CRGF
    • cripto
    • Cripto 1
    • Cripto 1 growth factor
    • Cripto-1 growth factor
    • Cripto1 growth factor
    • Epidermal growth factor like cripto protein CR1
    • Epidermal growth factor-like cripto protein CR1
    • TDGF 1
    • TDGF1
    • TDGF1_HUMAN
    • Teratocarcinoma derived growth factor 1
    • Teratocarcinoma-derived growth factor 1
    see all

  • Database links

    Images

    • Other - Human Cripto ELISA Kit (ab245719)
      Other - Human Cripto ELISA Kit (ab245719)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of human Cripto standard curve in Sample Diluent NS.
      Example of human Cripto standard curve in Sample Diluent NS.
      The Cripto standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
    • Interpolated concentrations of native Cripto in human NCCIT supernatant samples.
      Interpolated concentrations of native Cripto in human NCCIT supernatant samples.
      The concentrations of Cripto were measured in duplicates, interpolated from the Cripto standard curves and corrected for sample dilution. Undiluted samples are as follows: NCCIT supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cripto concentration was determined to be 1.58 ng/mL in NCCIT supernatant.
    • Interpolated concentrations of spiked Cripto in human serum and plasma samples.
      Interpolated concentrations of spiked Cripto in human serum and plasma samples.
      The concentrations of Cripto were measured in duplicates and interpolated from the Cripto standard curves. Undiluted samples are as follows: serum 12.5%, plasma (citrate) 12.5%, and plasma (EDTA) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
    • Sandwich ELISA - Human Cripto ELISA Kit (ab245719)
      Sandwich ELISA - Human Cripto ELISA Kit (ab245719)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Alternative products to Human Cripto ELISA Kit (ab245719)

    © 2021 Astana Biomed Group LLP. All rights reserved.