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Developmental Biology Lineage specification Ectoderm

Anti-5T4 antibody [EPR5530] (ab129058)

Price and availability

298 185 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-5T4 antibody [EPR5530] (ab129058)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5530] to 5T4
  • Suitable for: WB, IP
  • Reacts with: Rat, Human

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Overview

  • Product name

    Anti-5T4 antibody [EPR5530]
    See all 5T4 primary antibodies
  • Description

    Rabbit monoclonal [EPR5530] to 5T4
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Rat
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human 5T4 aa 350-450 (intracellular). The exact sequence is proprietary.

  • Positive control

    • HT 1376, HeLa, JAR and MCF7 cell lysates; MCF7 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5530
  • Isotype

    IgG
  • Research areas

    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Stem Cells
    • Lineage Markers
    • Endoderm
    • Stem Cells
    • Lineage Markers
    • Mesoderm
    • Cancer
    • Tumor immunology
    • Tumor-associated antigens
    • Developmental Biology
    • Lineage specification
    • Endoderm
    • Developmental Biology
    • Lineage specification
    • Ectoderm
    • Developmental Biology
    • Lineage specification
    • Mesoderm

Images

  • Western blot - Anti-5T4 antibody [EPR5530] (ab129058)
    Western blot - Anti-5T4 antibody [EPR5530] (ab129058)
    Anti-5T4 antibody [EPR5530] (ab129058) at 1/5000 dilution (purified) + Rat brain at 20 µg

    Secondary
    HRP goat-anti rabbit (H+L) at 1/1000 dilution

    Predicted band size: 46 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-5T4 antibody [EPR5530] (ab129058)
    Western blot - Anti-5T4 antibody [EPR5530] (ab129058)
    Anti-5T4 antibody [EPR5530] (ab129058) at 1/2000 dilution (unpurified) + Rat brain at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 46 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-5T4 antibody [EPR5530] (ab129058)
    Western blot - Anti-5T4 antibody [EPR5530] (ab129058)
    All lanes : Anti-5T4 antibody [EPR5530] (ab129058) at 1/5000 dilution (purified)

    Lane 1 : HT-1376 cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 46 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-5T4 antibody [EPR5530] (ab129058)
    Western blot - Anti-5T4 antibody [EPR5530] (ab129058)
    All lanes : Anti-5T4 antibody [EPR5530] (ab129058) at 1/2000 dilution (unpurified)

    Lane 1 : HT-1376 cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 46 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-5T4 antibody [EPR5530] (ab129058)
    Western blot - Anti-5T4 antibody [EPR5530] (ab129058)
    All lanes : Anti-5T4 antibody [EPR5530] (ab129058) at 1/1000 dilution (unpurified)

    Lane 1 : HT 1376 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : JAR cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 46 kDa

  • Immunoprecipitation - Anti-5T4 antibody [EPR5530] (ab129058)
    Immunoprecipitation - Anti-5T4 antibody [EPR5530] (ab129058)

    ab129058 (unpurified) at 1/50 immunoprecipitating 5T4 in MCF-7 (Lane 1). For western blotting, a HRP goat anti-rabbit was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunoprecipitation - Anti-5T4 antibody [EPR5530] (ab129058)
    Immunoprecipitation - Anti-5T4 antibody [EPR5530] (ab129058)

    ab129058 (unpurified) at 1/50 immunoprecipitating 5T4 in MCF-7 (Lane 1). For western blotting, a HRP goat anti-rabbit was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Anti-5T4 antibody [EPR5530] (ab129058)
    Anti-5T4 antibody [EPR5530] (ab129058)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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