Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-5T4 antibody [B3F1] - BSA and Azide free (ab255924)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [B3F1] to 5T4 - BSA and Azide free
  • Suitable for: Flow Cyt
  • Reacts with: Mouse

Overview

  • Product name

    Anti-5T4 antibody [B3F1] - BSA and Azide free
    See all 5T4 primary antibodies

  • Description

    Mouse monoclonal [B3F1] to 5T4 - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment within Mouse 5T4. The exact sequence is proprietary. Distal leucine rich repeat (LRR) extracellular domain.
    Database link: Q9Z0L0

  • Positive control

    • Flow Cyt: NIH/3T3 cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255924 is the carrier-free version of ab254168. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Flow Cytometry - Anti-5T4 antibody [B3F1] - BSA and Azide free (ab255924)
    Flow Cytometry - Anti-5T4 antibody [B3F1] - BSA and Azide free (ab255924)

    Flow cytometric analysis of RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage, Left) / NIH/3T3 (Mouse embryonic fibroblast, Right) labeling 5T4 with ab254168 at 1.012µg/ml (red) compared with Mouse monoclonal IgG Isotype Control (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Mouse IgG (Alexa Fluor® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody.

    Negative control: RAW 264.7 (PMID: 10366710).

    Gated on viable cells.

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA, (ab254168).

  • Anti-5T4 antibody [B3F1] - BSA and Azide free (ab255924)
    Anti-5T4 antibody [B3F1] - BSA and Azide free (ab255924)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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