Anti-5T4 antibody [B3F1] - BSA and Azide free (ab255924)
![Anti-5T4 antibody [B3F1] - BSA and Azide free (ab255924)](https://www.abcam.com/ps/products/255/ab255924/Images/ab255924-369260-Anti5T4-antibody-B3F1-flow-cytometry-raw2647-mouse.jpg "Anti-5T4 antibody [B3F1] - BSA and Azide free (ab255924)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [B3F1] to 5T4 - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Mouse
Overview
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Product name
Anti-5T4 antibody [B3F1] - BSA and Azide free
See all 5T4 primary antibodies -
Description
Mouse monoclonal [B3F1] to 5T4 - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Mouse
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Immunogen
Recombinant fragment within Mouse 5T4. The exact sequence is proprietary. Distal leucine rich repeat (LRR) extracellular domain.
Database link: Q9Z0L0 -
Positive control
- Flow Cyt: NIH/3T3 cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
ab255924 is the carrier-free version of ab254168. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
B3F1 -
Isotype
IgG2a -
Research areas
Images
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Flow Cytometry - Anti-5T4 antibody [B3F1] - BSA and Azide free (ab255924)
Flow cytometric analysis of RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage, Left) / NIH/3T3 (Mouse embryonic fibroblast, Right) labeling 5T4 with ab254168 at 1.012µg/ml (red) compared with Mouse monoclonal IgG Isotype Control (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Mouse IgG (Alexa Fluor® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody.
Negative control: RAW 264.7 (PMID: 10366710).
Gated on viable cells.
This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA, (ab254168).
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![Anti-5T4 antibody [B3F1] - BSA and Azide free (ab255924)](https://www.abcam.com/ps/products/255/ab255924/Images/ab255924-2-benefits-of-recombinant-antibodies.png)
Anti-5T4 antibody [B3F1] - BSA and Azide free (ab255924)
