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Developmental Biology Lineage specification Ectoderm

Anti-Anterior Gradient 2 antibody [EPR20164-278] - BSA and Azide free (ab251500)

Anti-Anterior Gradient 2 antibody [EPR20164-278] - BSA and Azide free (ab251500)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20164-278] to Anterior Gradient 2 - BSA and Azide free
  • Suitable for: ICC, Flow Cyt, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Anterior Gradient 2 antibody [EPR20164-278] - BSA and Azide free
    See all Anterior Gradient 2 primary antibodies
  • Description

    Rabbit monoclonal [EPR20164-278] to Anterior Gradient 2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251500 is the carrier-free version of ab209224. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251500 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    EPR20164-278
  • Isotype

    IgG
  • Research areas

    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Cancer
    • Tumor biomarkers
    • Other
    • Developmental Biology
    • Lineage specification
    • Ectoderm

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anterior Gradient 2 antibody [EPR20164-278] - BSA and Azide free (ab251500)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anterior Gradient 2 antibody [EPR20164-278] - BSA and Azide free (ab251500)

    This data was developed using ab209224, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Anterior Gradient 2 with ab209224 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human colon is observed [PMID: 24794000]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-Anterior Gradient 2 antibody [EPR20164-278] - BSA and Azide free (ab251500)
    Western blot - Anti-Anterior Gradient 2 antibody [EPR20164-278] - BSA and Azide free (ab251500)
    All lanes : Anti-Anterior Gradient 2 antibody [EPR20164-278] (ab209224) at 1/2000 dilution

    Lane 1 : Human AGR2 recombinant protein
    Lane 2 : Human AGR3 recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 19 kDa
    Observed band size: 17 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab209224, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 10 seconds; Lane 2: 3 minutes.

    Human AGR2 recombinant protein contains aa21-175 with a His-tag, and human AGR3 recombinant protein contains aa21-166 with GST-tag.

  • Western blot - Anti-Anterior Gradient 2 antibody [EPR20164-278] - BSA and Azide free (ab251500)
    Western blot - Anti-Anterior Gradient 2 antibody [EPR20164-278] - BSA and Azide free (ab251500)
    All lanes : Anti-Anterior Gradient 2 antibody [EPR20164-278] (ab209224) at 1/2000 dilution

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 2 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 19 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    This data was developed using ab209224, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Negative control: HeLa (PMID: 16551856).

  • Western blot - Anti-Anterior Gradient 2 antibody [EPR20164-278] - BSA and Azide free (ab251500)
    Western blot - Anti-Anterior Gradient 2 antibody [EPR20164-278] - BSA and Azide free (ab251500)
    Lanes 1-3 : Anti-Anterior Gradient 2 antibody [EPR20164-278] (ab209224) at 1/1000 dilution
    Lane 4 : Anti-Anterior Gradient 2 antibody [EPR20164-278] (ab209224) at 1/2000 dilution

    Lane 1 : Mouse stomach lysate
    Lane 2 : Rat stomach lysate
    Lane 3 : Rat small intestine lysate
    Lane 4 : Human stomach lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lane 4 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 19 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?



    This data was developed using ab209224, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-3: 3 minutes; Lane 4: 10 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anterior Gradient 2 antibody [EPR20164-278] - BSA and Azide free (ab251500)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anterior Gradient 2 antibody [EPR20164-278] - BSA and Azide free (ab251500)

    This data was developed using ab209224, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue labeling Anterior Gradient 2 with ab209224 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human ovary cancer is observed [PMID: 22752467]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anterior Gradient 2 antibody [EPR20164-278] - BSA and Azide free (ab251500)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anterior Gradient 2 antibody [EPR20164-278] - BSA and Azide free (ab251500)

    This data was developed using ab209224, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Anterior Gradient 2 with ab209224 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on mouse stomach is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anterior Gradient 2 antibody [EPR20164-278] - BSA and Azide free (ab251500)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anterior Gradient 2 antibody [EPR20164-278] - BSA and Azide free (ab251500)
    This data was developed using ab209224, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Anterior Gradient 2 with ab209224 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on rat stomach is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anterior Gradient 2 antibody [EPR20164-278] - BSA and Azide free (ab251500)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anterior Gradient 2 antibody [EPR20164-278] - BSA and Azide free (ab251500)
    This data was developed using ab209224, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Anterior Gradient 2 with ab209224 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative control: human pancreas [PMID: 20550709]. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-Anterior Gradient 2 antibody [EPR20164-278] - BSA and Azide free (ab251500)
    Immunocytochemistry - Anti-Anterior Gradient 2 antibody [EPR20164-278] - BSA and Azide free (ab251500)
    This data was developed using ab209224, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) and HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Anterior Gradient 2 with ab209224 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cell line. Negative control: HeLa (PMID: 16551856). The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
  • Flow Cytometry - Anti-Anterior Gradient 2 antibody [EPR20164-278] - BSA and Azide free (ab251500)
    Flow Cytometry - Anti-Anterior Gradient 2 antibody [EPR20164-278] - BSA and Azide free (ab251500)
    This data was developed using ab209224, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling Anterior Gradient 2 with ab209224 at 1/70 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
  • Flow Cytometry - Anti-Anterior Gradient 2 antibody [EPR20164-278] - BSA and Azide free (ab251500)
    Flow Cytometry - Anti-Anterior Gradient 2 antibody [EPR20164-278] - BSA and Azide free (ab251500)
    This data was developed using ab209224, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Anterior Gradient 2 with ab209224 at 1/70 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. Negative control: HeLa
  • Anti-Anterior Gradient 2 antibody [EPR20164-278] - BSA and Azide free (ab251500)
    Anti-Anterior Gradient 2 antibody [EPR20164-278] - BSA and Azide free (ab251500)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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