Anti-KAT9 / Elp3 antibody [EPR18472] - BSA and Azide free (ab251047)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18472] to KAT9 / Elp3 - BSA and Azide free
- Suitable for: ICC, IP, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-KAT9 / Elp3 antibody [EPR18472] - BSA and Azide free
See all KAT9 / Elp3 primary antibodies -
Description
Rabbit monoclonal [EPR18472] to KAT9 / Elp3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251047 is the carrier-free version of ab190907. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251047 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
EPR18472 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907) at 1/5000 dilution
Lanes 1 & 7 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lane 4 : 293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lane 5 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size: 62 kDa
Observed band size: 62 kDa
Exposure time: 5 secondsThis data was developed using ab190907, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907) at 1/2000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : Human fetal heart tissue lysate
Lane 3 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 62 kDa
Observed band size: 62 kDa
Exposure time: 3 minutesThis data was developed using ab190907, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907) at 1/2000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse spleen tissue lysate
Lane 5 : Rat brain tissue lysate
Lane 6 : Rat heart tissue lysate
Lane 7 : Rat kidney tissue lysate
Lane 8 : Rat spleen tissue lysate
Lane 9 : C6 (Rat glial tumor cells) whole cell lysate
Lane 10 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 11 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 12 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size: 62 kDa
Observed band size: 62 kDa
Exposure time: 5 secondsThis data was developed using ab190907, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907) at 1/10000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cytosolic fraction lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) nuclear fraction lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size: 62 kDa
Observed band size: 62 kDa
Exposure time: 15 secondsThis data was developed using ab190907, the same antibody clone in a different buffer formulation.
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This data was developed using ab190907, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling KAT9 / Elp3 with ab190907 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).Confocal image showing cytoplasmic and nuclear staining on HeLa cells line.The staining pattern is in agreement with the data shown in the following publications: Cell 2009. 136, 551-64 (PMID: 19185337) and J Biol Chem. 2002. 277, 3047-3052 (PMID: 11714725). The nuclear counterstain is DAPI (blue).Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).The negative controls are as follows:-
-ve control 1: ab190907 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution. -
This data was developed using ab190907, the same antibody clone in a different buffer formulation.KAT9 / Elp3 was immunoprecipitated from 1mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate with ab190907 at 1/70 dilution. Lane 1: MCF7 whole cell lysate 10ug (Input).Lane 2: ab190907 IP in MCF7 whole cell lysate.Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab190907 in MCF7 whole cell lysate.Western blot was performed from the immunoprecipitate using ab190907 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 3 seconds.
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