Anti-DDX4 / MVH antibody (ab13840)
Key features and details
- Rabbit polyclonal to DDX4 / MVH
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-DDX4 / MVH antibody
See all DDX4 / MVH primary antibodies -
Description
Rabbit polyclonal to DDX4 / MVH -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-Fr MouseIHC-P HumanWB Mouse
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Immunogen
Images
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Immunohistochemistry (Frozen sections) - Anti-DDX4 / MVH antibody (ab13840) This image is courtesy of Petra Hajkova, Gurdon Institute, University of Cambridge
Mouse embryonic gonad cells were fixed with 4% PFA, permeabilised and blocked with PBS, 1% BSA, 0.1% TX-100 and incubated with the DDX4 antibody, ab13840 (1
µ The image shows that the anti-DDX4 antibody, ab13840, stains the cytoplasm of mouse primordial germ cells when used in IHC-Fr.
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Western blot - Anti-DDX4 / MVH antibody (ab13840)All lanes : Anti-DDX4 / MVH antibody (ab13840) at 1 µg/ml
Lane 1 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
Lane 2 : Human testis tissue lysate - total protein (ab30257)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 76 kDa
Additional bands at: 28 kDa, 47 kDa. We are unsure as to the identity of these extra bands.
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Western blot - Anti-DDX4 / MVH antibody (ab13840)All lanes : ab13840 at 1 µg/ml
Lane 1 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
Lane 2 : Human testis tissue lysate - total protein
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 79 kDa why is the actual band size different from the predicted?
Additional bands at: 47 kDa, 52 kDa. We are unsure as to the identity of these extra bands.
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Western blot - Anti-DDX4 / MVH antibody (ab13840)All lanes : Anti-DDX4 / MVH antibody (ab13840) at 1 µg/ml
Lane 1 : Mouse Ovary Lysate at 10 µg
Lane 2 : Mouse Embryonic Germ Cell Lysate at 20 µg
Lane 3 : Mouse Ovary Lysate at 10 µg withDDX4 / MVH peptide (ab13841) at 1 µg/ml
Lane 4 : Mouse Embryonic Germ Cell Lysate at 20 µg withDDX4 / MVH peptide (ab13841) at 1 µg/ml
Predicted band size: 76 kDa
Observed band size: 76 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody (ab13840)IHC image of DDX4/MVH staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13840, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
