Anti-Syntaxin 16 antibody [EPR9156] (ab134945)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9156] to Syntaxin 16
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Syntaxin 16 antibody [EPR9156]
See all Syntaxin 16 primary antibodies -
Description
Rabbit monoclonal [EPR9156] to Syntaxin 16 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human Syntaxin 16 aa 50-150. The exact sequence is proprietary.
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Positive control
- Human testis and lung adenocarcinoma tissues; HepG2 cells; 293T, HeLa, HepG2 and SH-SY5Y cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9156 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Syntaxin 16 antibody [EPR9156] (ab134945) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 37 kDa
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Immunofluorescence staining of HepG2 cells, labelling Syntaxin 16 using ab134945 at a 1/100 dilution (red). Cell nuclei are shown in blue.
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Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human lung adenocarcinoma tissue, labelling Syntaxin 16 using ab134945 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human testis tissue, labelling Syntaxin 16 using ab134945 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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