Anti-Syntaxin 16 antibody - Drosophila Golgi/Trans-Golgi Marker (ab32340)
Key features and details
- Rabbit polyclonal to Syntaxin 16 - Drosophila Golgi/Trans-Golgi Marker
- Suitable for: WB
- Reacts with: Drosophila melanogaster
- Isotype: IgG
Overview
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Product name
Anti-Syntaxin 16 antibody - Drosophila Golgi/Trans-Golgi Marker
See all Syntaxin 16 primary antibodies -
Description
Rabbit polyclonal to Syntaxin 16 - Drosophila Golgi/Trans-Golgi Marker -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Drosophila melanogaster
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Immunogen
Synthetic peptide corresponding to Drosophila melanogaster Syntaxin 16 aa 200-300 conjugated to keyhole limpet haemocyanin.
(Peptide available asab32339)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB Drosophila melanogasterApplication Abreviews Notes WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).Notes WB
Use a concentration of 2.5 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).Application notesIs unsuitable for ICC/IF.Target
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Function
SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Genetic variations in STX16 may be a cause of pseudohypoparathyroidism type 1B (PHP1B) [MIM:603233]. Pseudohypoparathyroidism refers to a heterogeneous group of disorders characterized by resistance to parathyroid hormone (PTH). PHP1B is characterized by PTH-resistant hypocalcemia and hyperphosphatemia. Patients affected with PHP1B lack developmental defects characteristic of Albright hereditary osteodystrophy, and typically show no other endocrine abnormalities besides resistance to PTH. In some cases microdeletions involving STX16 appear to cause loss of methylation at exon A/B of the GNAS gene, resulting in PHP1B. -
Sequence similarities
Belongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain. -
Cellular localization
Cytoplasm and Golgi apparatus membrane. - Information by UniProt
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Alternative names
- hsyn16 antibody
- MGC90328 antibody
- Stx16 antibody
see all
Images
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Western blot - Anti-Syntaxin 16 antibody - Drosophila Golgi/Trans-Golgi Marker (ab32340)Anti-Syntaxin 16 antibody - Drosophila Golgi/Trans-Golgi Marker (ab32340) at 1 µg/ml +
Schneider L2 whole cell lysate (ab14893) at 20 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Datasheets and documents
References (4)
ab32340 has been referenced in 4 publications.
- Bellec K et al. Stratum recruits Rab8 at Golgi exit sites to regulate the basolateral sorting of Notch and Sanpodo. Development 145:N/A (2018). PubMed: 29967125
- Charng WL et al. Drosophila Tempura, a novel protein prenyltransferase a subunit, regulates notch signaling via Rab1 and Rab11. PLoS Biol 12:e1001777 (2014). ICC/IF ; Drosophila melanogaster . PubMed: 24492843
- Hornbruch-Freitag C et al. Drosophila Swiprosin-1/EFHD2 accumulates at the prefusion complex stage during Drosophila myoblast fusion. J Cell Sci 124:3266-78 (2011). ICC/IF ; Drosophila melanogaster . PubMed: 21896648
- Tiklová K et al. Epithelial septate junction assembly relies on melanotransferrin iron binding and endocytosis in Drosophila. Nat Cell Biol 12:1071-7 (2010). WB, IHC-P ; Drosophila melanogaster . PubMed: 20935638
Images
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Western blot - Anti-Syntaxin 16 antibody - Drosophila Golgi/Trans-Golgi Marker (ab32340)Anti-Syntaxin 16 antibody - Drosophila Golgi/Trans-Golgi Marker (ab32340) at 1 µg/ml +
Schneider L2 whole cell lysate (ab14893) at 20 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
