Anti-IDI1 + IDI2 antibody [EPR17213] (ab205617)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17213] to IDI1 + IDI2
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-IDI1 + IDI2 antibody [EPR17213]
See all IDI1+IDI2 primary antibodies -
Description
Rabbit monoclonal [EPR17213] to IDI1 + IDI2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2, HeLa and MOLT-4 whole cell lysates; Human fetal liver, fetal kidney, fetal skeletal muscle lysate, colon cancer lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17213 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-IDI1 + IDI2 antibody [EPR17213] (ab205617) at 1/5000 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with IDI1 peptide
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with IDI2 peptide
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 3 minutesBlocking and diluting buffer was 5% NFDM /TBST.
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Anti-IDI1 + IDI2 antibody [EPR17213] (ab205617) at 1/5000 dilution + Human fetal liver lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 3 minutesBlocking and diluting buffer was 5% NFDM /TBST.
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All lanes : Anti-IDI1 + IDI2 antibody [EPR17213] (ab205617) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal skeletal muscle lysate
Lane 3 : Human colon cancer lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 3 minutesBlocking and diluting buffer was 5% NFDM /TBST
-
All lanes : Anti-IDI1 + IDI2 antibody [EPR17213] (ab205617) at 1/5000 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 2 minutesBlocking and diluting buffer was 5% NFDM /TBST
Exposure time Lane 1 and 2: 2minutes;
Lane 3: 3minutes -