Anti-Histone H4 (butyryl K16) antibody (ab240615)
Key features and details
- Rabbit polyclonal to Histone H4 (butyryl K16)
- Suitable for: WB, ICC/IF, ChIP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Histone H4 (butyryl K16) antibody
See all Histone H4 primary antibodies -
Description
Rabbit polyclonal to Histone H4 (butyryl K16) -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, ChIPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Histone H4 (butyryl K16).
Database link: P62805 -
Positive control
- WB: HEK-293 and K562 whole cell lysates treated with 30mM sodium butyrate for 4 hours. ICC/IF: HeLa cells treated with 30mM sodium butyrate for 4 hours. ChIP: Chromatin prepared from HeLa cells treated with 30mM sodium butyrate for 4 hours.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Images
-
Western blot - Anti-Histone H4 (butyryl K16) antibody (ab240615)All lanes : Anti-Histone H4 (butyryl K16) antibody (ab240615) at 1/100 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 3 : HEK-293 whole cell lysate, untreated (-)
Lane 4 : K562 whole cell lysate, untreated (-)
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 11 kDa
-
Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (butyryl K16) antibody (ab240615)HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 30 mM sodium butyrate for 4 hours) labeling Histone H4 (butyryl K16) with ab240615 at 1/50 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
-
ChIP - Anti-Histone H4 (butyryl K16) antibody (ab240615)HeLa (human epithelial cell line from cervix adenocarcinoma; 4 x 106, treated with 30mM sodium butyrate for 4 hours) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5 μg ab240615 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
