Mouse and Rabbit Specific HRP Plus (ABC) Detection IHC Kit (Ready To Use) (ab93697)
Overview
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Product name
Mouse and Rabbit Specific HRP Plus (ABC) Detection IHC Kit (Ready To Use)
See all Polyvalent HRP kits -
Product overview
The reagents in this kit constitute a labeled streptavidin-biotin immunoenzymatic antigen detection system with increased sensitivity. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.
Reacts with Mouse and Rabbit IgG (H+L)
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Tested applications
Suitable for: IHC-Pmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 60 ml 125 ml Biotinylated Goat Anti-Polyvalent Plus 1 x 60ml 1 x 125ml Protein block 1 x 60ml 1 x 125ml Streptavidin Peroxidase Plus 1 x 60ml 1 x 125ml -
Research areas
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Relevance
Polyvalent HRP is a labeled streptavidin-biotin immunoenzymatic antigen detection system. Horseradish peroxidase (HRP) readily combines with hydrogen peroxide (H2O2) and the resultant complex can oxidize a wide variety of chromogenic substrates. -
Alternative names
- Polyvalent/HRP
Images
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Mouse and Rabbit Specific HRP Plus (ABC) Detection IHC Kit (Ready To Use) Image from McGee-Lawrence ME et al., Biochem Biophys Res Commun. 2013;411(4):886-90. Fig 3.; doi: 10.1016/j.bbrc.2013.10.155 with permission from Elsevier.
Immunohistochemistry analysis of mouse femur tissue sections. Femurs were fixed in 10% neutral buffered formalin and decalcified for 7 days in 15% EDTA; complete decalcification was confirmed by X-ray. Decalcified bones were embedded in paraffin and sectioned longitudinally through the defect at a thickness of 8 microns. Immunohistochemical staining was performed with monoclonal anti-activated beta-catenin (un-phosphorylated on Ser33/37 and Thr41), or an IgG isotype control. Chromogens were developed using ab93697, Mouse and Rabbit Specific HRP Plus (ABC) Detection IHC Kit (Ready to Use), followed by 3′-3′-diaminobenzidine (DAB), and then counterstained with fast green. Beta-catenin levels are elevated in defects of Sost-/- mice.
