Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Rabbit polyclonal to YB1
Suitable for: IHC-P, WB, IP
Reacts with: Mouse, Human
Isotype: IgG

Product name

Anti-YB1 antibody
See all YB1 primary antibodies
Description

Rabbit polyclonal to YB1
Host species

Rabbit
Tested applications

Suitable for: IHC-P, WB, IPmore details
Species reactivity

Reacts with: Mouse, Human
Predicted to work with: Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pigeon, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Immunogen

Synthetic peptide, corresponding to a region within amino acids 224-274 of Human YB1 (NP_004550).
Positive control
- Hela, Jurkat and 293T cell lysates

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate

pH 7 to 8
Concentration information loading...
Purity

Immunogen affinity purified
Purification notes

ab114999 was affinity purified using an epitope specific to YB1 immobilized on solid support.
Clonality

Polyclonal
Isotype

IgG
Research areas

Western blot - Anti-YB1 antibody (ab114999)

All lanes : Anti-YB1 antibody (ab114999) at 0.1 µg/ml

Lane 1 : Hela cell lysate at 50 µg
Lane 2 : Hela cell lysate at 15 µg
Lane 3 : 293T cell lysate at 50 µg
Lane 4 : Jurkat cell lysate at 50 µg

Developed using the ECL technique.

Predicted band size: 36 kDa

Exposure time: 30 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YB1 antibody (ab114999)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal cell carcinoma tissue labelling YB1 with ab114999 at 1/1000 (1µg/ml). Detection: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YB1 antibody (ab114999)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach linitis plastica tissue labelling YB1 with ab114999 at 1/5000 (0.2µg/ml). Detection: DAB.
Immunoprecipitation - Anti-YB1 antibody (ab114999)

Immunoprecipitation : 1 mg of Hela cell lysate immunoprecipitated using ab11499 at 6 µg/mg lysate; 20% of immunoprecipitate loaded per lane
Western Blot: ab114999 at 1 µg/ml

developed using the ECL technique

Exposure time: 3 seconds

Predicted band size : 36 kDa

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