Anti-YB1 antibody (ab114999)
Key features and details
- Rabbit polyclonal to YB1
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-YB1 antibody
See all YB1 primary antibodies -
Description
Rabbit polyclonal to YB1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pigeon, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 224-274 of Human YB1 (NP_004550).
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Positive control
- Hela, Jurkat and 293T cell lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab114999 was affinity purified using an epitope specific to YB1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-YB1 antibody (ab114999)All lanes : Anti-YB1 antibody (ab114999) at 0.1 µg/ml
Lane 1 : Hela cell lysate at 50 µg
Lane 2 : Hela cell lysate at 15 µg
Lane 3 : 293T cell lysate at 50 µg
Lane 4 : Jurkat cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 36 kDa
Exposure time: 30 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YB1 antibody (ab114999)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal cell carcinoma tissue labelling YB1 with ab114999 at 1/1000 (1µg/ml). Detection: DAB. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YB1 antibody (ab114999)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach linitis plastica tissue labelling YB1 with ab114999 at 1/5000 (0.2µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-YB1 antibody (ab114999)Immunoprecipitation : 1 mg of Hela cell lysate immunoprecipitated using ab11499 at 6 µg/mg lysate; 20% of immunoprecipitate loaded per laneWestern Blot: ab114999 at 1 µg/ml
developed using the ECL technique
Exposure time: 3 seconds
Predicted band size : 36 kDa
