Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10402(B)] to TMS1/ASC
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-TMS1/ASC antibody [EPR10402(B)]
    See all TMS1/ASC primary antibodies

  • Description

    Rabbit monoclonal [EPR10402(B)] to TMS1/ASC

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human TMS1/ASC. The exact sequence is proprietary.

  • Positive control

    • THP1, MCF7, U937 cell lysates; THP1 cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as TMS1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700)
    Western blot - Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700)
    All lanes : Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700) at 1/5000 dilution (purified)

    Lane 1 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates
    Lane 2 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 22 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700)
    Western blot - Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2:     TMS1/ASC knockout HAP1 cell lysate (20 µg)
    Lane 3: U937 cell lysate (20 µg)
    Lane 4: THP1 cell lysate (20 µg)
    Lanes 1 - 4:     Merged signal (red and green). Green - ab151700 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

    Unpurified ab151700 was shown to specifically react with TMS1/ASC when TMS1/ASC knockout samples were used. Wild-type and TMS1/ASC knockout samples were subjected to SDS-PAGE. Unpurified ab151700 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700)
    Immunoprecipitation - Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700)

    ab151700 (purified) at 1:30 dilution (2ug) immunoprecipitating TMS1/ASC in THP-1 (Human monocytic leukemia monocyte) whole cell lysate.
    Lane 1 (input): THP-1 (Human monocytic leukemia monocyte) whole cell lysate 10ug
    Lane 2 (+): ab151700 & THP-1 (Human monocytic leukemia monocyte) whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab151700 in THP-1 (Human monocytic leukemia monocyte) whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700)
    Western blot - Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700)
    All lanes : Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700) at 1/1000 dilution (unpurified)

    Lane 1 : THP1 cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : U937 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 22 kDa

  • Flow Cytometry - Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700)
    Flow Cytometry - Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700)

    Flow cytometric analysis of permeabilized THP1 cells labeling TMS1/ASC with unpurified ab151700 at 1/10 dilution (red). Rabbit IgG negative control (green).

  • Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700)
    Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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