Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TIM44 antibody [EPR16821] - BSA and Azide free (ab251197)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16821] to TIM44 - BSA and Azide free
  • Suitable for: WB, ICC, IP, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-TIM44 antibody [EPR16821] - BSA and Azide free
    See all TIM44 primary antibodies

  • Description

    Rabbit monoclonal [EPR16821] to TIM44 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251197 is the carrier-free version of ab194829. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251197 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody [EPR16821] - BSA and Azide free (ab251197)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody [EPR16821] - BSA and Azide free (ab251197)
    This data was developed using ab194829, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling TIM44 with ab194829 at 1/100 dilution (5μg/ml). A Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution was used as secondary (ab97051). Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab194829. Cytoplasm staining on humanendometrial adenocarcinoma was observed. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-TIM44 antibody [EPR16821] - BSA and Azide free (ab251197)
    Western blot - Anti-TIM44 antibody [EPR16821] - BSA and Azide free (ab251197)
    Anti-TIM44 antibody [EPR16821] (ab194829) at 1/1000 dilution + mouse heart tissue lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa



    This data was developed using ab194829, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-TIM44 antibody [EPR16821] - BSA and Azide free (ab251197)
    Immunocytochemistry - Anti-TIM44 antibody [EPR16821] - BSA and Azide free (ab251197)
    This data was developed using ab194829, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of A431 cells labeling TIM44 with ab194829 at 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. Counterstain: DAPI. Cytoplasm staining on A431 cell line was observed.
  • Western blot - Anti-TIM44 antibody [EPR16821] - BSA and Azide free (ab251197)
    Western blot - Anti-TIM44 antibody [EPR16821] - BSA and Azide free (ab251197)
    All lanes : Anti-TIM44 antibody [EPR16821] (ab194829) at 1/5000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa



    This data was developed using ab194829, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-TIM44 antibody [EPR16821] - BSA and Azide free (ab251197)
    Immunoprecipitation - Anti-TIM44 antibody [EPR16821] - BSA and Azide free (ab251197)
    This data was developed using ab194829, the same antibody clone in a different buffer formulation.Immunoprecipitation analysis of immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab194826 at 1/20 dilution (Lane 1). Lane 2: PBS instead of K562 cell lysate. ab194829 at 1/1000 was used for subsequent western blot detection. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.
  • Anti-TIM44 antibody [EPR16821] - BSA and Azide free (ab251197)
    Anti-TIM44 antibody [EPR16821] - BSA and Azide free (ab251197)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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