Antibodies, Proteins, Kits and Reagents for Life Science

Anti-TMS1/ASC antibody [EPR10403] - BSA and Azide free (ab242398)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10403] to TMS1/ASC - BSA and Azide free
Suitable for: WB, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-TMS1/ASC antibody [EPR10403] - BSA and Azide free
See all TMS1/ASC primary antibodies
Description

Rabbit monoclonal [EPR10403] to TMS1/ASC - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide within Human TMS1/ASC aa 100 to the C-terminus. The exact sequence is proprietary.
Database link: Q9ULZ3
Positive control
- WB: THP-1 and U937 cell lysates. ICC/IF: HeLa and THP-1 cells. Flow Cyt: THP-1 cells.
General notes
ab242398 is the carrier-free version of ab155970 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab242398 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as TMS1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR10403
Isotype

IgG
Research areas

Flow Cytometry - Anti-TMS1/ASC antibody [EPR10403] - BSA and Azide free (ab242398)

ab155970 staining TMS1/ASC in the human cell line THP-1 (Human monocytic leukemia cell line) by flow cytometry.

Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/50. A goat anti rabbit IgG (Alexa Fluor^® 488) at a dilution of 1/2000 was used as the secondary antibody.

Isoytype control: Rabbit monoclonal IgG (Black)

Unlabeled control: Cell without incubation with primary antibody and secondary antibody (Blue)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155970)
Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody [EPR10403] - BSA and Azide free (ab242398)

ab133645 staining TMS1/ASC in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence).

Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab195889 was used as a counterstain for primary antibody ab155970 at 1/200. DAPI was used as a nuclear counterstain and PBS as a negative control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155970)
Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody [EPR10403] - BSA and Azide free (ab242398)

Immunofluorescence analysis of THP-1 (Human monocytic leukemia cell line) cells, labeling TMS1/ASC using ab155970 at a 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155970).
Anti-TMS1/ASC antibody [EPR10403] - BSA and Azide free (ab242398)

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