Anti-DENN antibody [EPR4919] (ab134117)
![Anti-DENN antibody [EPR4919] (ab134117)](https://www.abcam.com/ps/products/134/ab134117/Images/ab134117-148318-anti-denn-antibody-epr4919-western-blot.jpg "Anti-DENN antibody [EPR4919] (ab134117)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4919] to DENN
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Overview
-
Product name
Anti-DENN antibody [EPR4919]
See all DENN primary antibodies -
Description
Rabbit monoclonal [EPR4919] to DENN -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
-
Immunogen
Synthetic peptide within Human DENN (C terminal). The exact sequence is proprietary.
-
Positive control
- HeLa cell lysate, 293T cell lysate, K562 cell lysate, NIH 3T3 cell lysate, Human breast tissue.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 7.00 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4919 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-DENN antibody [EPR4919] (ab134117)All lanes : Anti-DENN antibody [EPR4919] (ab134117) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : K562 cell lysate
Lane 4 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 183 kDa
Observed band size: 220 kDa why is the actual band size different from the predicted?
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DENN antibody [EPR4919] (ab134117)](https://www.abcam.com/ps/products/134/ab134117/Images/ab134117-148317-anti-denn-antibody-epr4919-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DENN antibody [EPR4919] (ab134117)Immunohistochemical analysis of paraffin embedded Human breast tissue labelling DENN with ab134117 at 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![OI-RD Scanning - Anti-DENN antibody [EPR4919] (ab134117)](https://www.abcam.com/ps/products/134/ab134117/Images/ab134117-148316-anti-denn-antibody-epr4919.jpg)
OI-RD Scanning - Anti-DENN antibody [EPR4919] (ab134117) -
![Anti-DENN antibody [EPR4919] (ab134117)](https://www.abcam.com/ps/products/134/ab134117/Images/ab134117-4-benefits-of-recombinant-antibodies.png)
Anti-DENN antibody [EPR4919] (ab134117)
