Anti-GODZ/DHHC-3 antibody (ab31837)
Key features and details
- Rabbit polyclonal to GODZ/DHHC-3
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-GODZ/DHHC-3 antibody -
Description
Rabbit polyclonal to GODZ/DHHC-3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Previously labelled as GODZ.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab31837 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseAll applications RatHumanXenopus laevisApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).Target
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Function
Palmitoyltransferase with broad specificity. Palmitoylates GABA receptors on their gamma subunit (GABRG1, GABRG2 and GABRG3), which regulates synaptic clustering and/or cell surface stability. Palmitoylates glutamate receptors GRIA1 and GRIA2, which leads to their retention in Golgi. -
Sequence similarities
Belongs to the DHHC palmitoyltransferase family.
Contains 1 DHHC-type zinc finger. -
Domain
The DHHC domain is required for palmitoyltransferase activity. -
Post-translational
modificationsAutopalmitoylated. -
Cellular localization
Golgi apparatus membrane. - Information by UniProt
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Database links
- Entrez Gene: 51304 Human
- Entrez Gene: 69035 Mouse
- Entrez Gene: 301081 Rat
- SwissProt: Q9NYG2 Human
- SwissProt: Q8R173 Mouse
- Unigene: 61430 Human
- Unigene: 28300 Mouse
- Unigene: 8573 Rat
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Alternative names
- DHHC-3 antibody
- GABA-A receptor-associated membrane protein 1 antibody
- GODZ antibody
see all
Images
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Western blot - Anti-GODZ/DHHC-3 antibody (ab31837)Anti-GODZ/DHHC-3 antibody (ab31837) at 1 µg/ml + Cerebellum (Mouse) Tissue Lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 34 kDa
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
Protocols
Datasheets and documents
References (8)
ab31837 has been referenced in 8 publications.
- Mukherjee A et al. Specific Palmitoyltransferases Associate with and Activate the Epithelial Sodium Channel. J Biol Chem 292:4152-4163 (2017). PubMed: 28154191
- Kilpatrick CL et al. Dissociation of Golgi-associated DHHC-type Zinc Finger Protein (GODZ)- and Sertoli Cell Gene with a Zinc Finger Domain-ß (SERZ-ß)-mediated Palmitoylation by Loss of Function Analyses in Knock-out Mice. J Biol Chem 291:27371-27386 (2016). PubMed: 27875292
- Lievens PM et al. ZDHHC3 Tyrosine Phosphorylation Regulates Neural Cell Adhesion Molecule Palmitoylation. Mol Cell Biol 36:2208-25 (2016). WB ; Mouse . PubMed: 27247265
- Lai J & Linder ME Oligomerization of DHHC protein S-acyltransferases. J Biol Chem 288:22862-70 (2013). PubMed: 23793055
- Levy AD et al. Subcellular Golgi localization of stathmin family proteins is promoted by a specific set of DHHC palmitoyl transferases. Mol Biol Cell 22:1930-42 (2011). PubMed: 21471001
- Shmueli A et al. Ndel1 palmitoylation: a new mean to regulate cytoplasmic dynein activity. EMBO J 29:107-19 (2010). WB, ICC/IF ; Human . PubMed: 19927128
- Noritake J et al. Mobile DHHC palmitoylating enzyme mediates activity-sensitive synaptic targeting of PSD-95. J Cell Biol 186:147-60 (2009). WB, ICC/IF ; Rat . PubMed: 19596852
- Tsutsumi R et al. Identification of G protein alpha subunit-palmitoylating enzyme. Mol Cell Biol 29:435-47 (2009). WB, ICC/IF ; Human . PubMed: 19001095
Images
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Western blot - Anti-GODZ/DHHC-3 antibody (ab31837)Anti-GODZ/DHHC-3 antibody (ab31837) at 1 µg/ml + Cerebellum (Mouse) Tissue Lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 34 kDa
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute