Anti-TRIM4 antibody - N-terminal (ab229952)
Key features and details
- Rabbit polyclonal to TRIM4 - N-terminal
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-TRIM4 antibody - N-terminal
See all TRIM4 primary antibodies -
Description
Rabbit polyclonal to TRIM4 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment corresponding to Human TRIM4 aa 1-150 (N terminal).
Database link: Q9C037 -
Positive control
- WB: MCF7, HeLa and A459 whole cell lysates; Rat brain and heart tissue lysates. IHC-P: Human colon cancer tissue. ICC/IF: HepG2 cells.
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General notes
Previously labelled as RNF87.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TRIM4 antibody - N-terminal (ab229952)Lanes 1-4 : Anti-TRIM4 antibody - N-terminal (ab229952) at 1/500 dilution
Lane 5 : Anti-TRIM4 antibody - N-terminal (ab229952) at 500 µg/ml
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : Rat heart tissue lysate
Secondary
All lanes : Goat anti-rabbit IgG at 1/50000 dilution
Predicted band size: 57,54,34 kDa
Observed band size: 35,55,62,90 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM4 antibody - N-terminal (ab229952)Paraffin-embedded human colon cancer tissue stained for TRIM4 using ab229952 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-TRIM4 antibody - N-terminal (ab229952)HepG2 (human liver hepatocellular carcinoma cell line) cells stained for TRIM4 (green) using ab229952 at 1/100 dilution in ICC/IF, followed by Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG(H+L) as secondary antibody.
