Anti-RNF21/IFP1 antibody [EPR11305(2)] (ab180130)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11305(2)] to RNF21/IFP1
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-RNF21/IFP1 antibody [EPR11305(2)]
See all RNF21/IFP1 primary antibodies -
Description
Rabbit monoclonal [EPR11305(2)] to RNF21/IFP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human -
Immunogen
Synthetic peptide within Human RNF21/IFP1 aa 50-150. The exact sequence is proprietary.
Database link: Q9BYJ4 -
Positive control
- K562 cells; K562 and Jurkat cell lysate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as RNF21
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11305(2) -
Isotype
IgG -
Research areas
Images
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Anti-RNF21/IFP1 antibody [EPR11305(2)] (ab180130) at 1/2000 dilution + Jurkat cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 57 kDa
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Flow cytometry analysis of K562 cells labeling RNF21/IFP1 using ab180130 at 1/60 dilution. A Goat anti rabbit IgG (FITC) at 1/75 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit IgG.
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Anti-RNF21/IFP1 antibody [EPR11305(2)] (ab180130) at 1/10000 dilution + K562 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 57 kDa
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