Flow cytometric analysis of Semaphorin 4D/CD100 expression on mouse BABL/c splenocytes. Cells were stained with ab277139 at 1/25 dilution, then a FITC-conjugated second step antibody (Black). Cells were also staibed with an isotype control (Grey). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Immunofluorescence staining of Semaphorin 4D/CD100 in mouse spleen cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with ab277139 at 1/60 dilution at 37°C for 1 hour. Then cells were stained with the Alexa Fluor^® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane.