Anti-TBP-1 antibody [EPR12153] (ab171974)
![Anti-TBP-1 antibody [EPR12153] (ab171974)](https://www.abcam.com/ps/products/171/ab171974/Images/ab171974-194485-anti-tbp-1-antibody-epr12153-western-blot.jpg "Anti-TBP-1 antibody [EPR12153] (ab171974)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12153] to TBP-1
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-TBP-1 antibody [EPR12153]
See all TBP-1 primary antibodies -
Description
Rabbit monoclonal [EPR12153] to TBP-1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human TBP-1 aa 400 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: P17980 -
Positive control
- 293T, HeLa and HepG2 cell lysates; Human pancreas tissue; Human Tonsil tissue; Permeabilized HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12153 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TBP-1 antibody [EPR12153] (ab171974)All lanes : Anti-TBP-1 antibody [EPR12153] (ab171974) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 49 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBP-1 antibody [EPR12153] (ab171974)](https://www.abcam.com/ps/products/171/ab171974/Images/ab171974-194483-anti-tbp-1-antibody-epr12153-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBP-1 antibody [EPR12153] (ab171974)Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling TBP-1 with ab171974 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBP-1 antibody [EPR12153] (ab171974)](https://www.abcam.com/ps/products/171/ab171974/Images/ab171974-194484-anti-tbp-1-antibody-epr12153-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBP-1 antibody [EPR12153] (ab171974)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling TBP-1 with ab171974 at 1/100 dilution
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-TBP-1 antibody [EPR12153] (ab171974)](https://www.abcam.com/ps/products/171/ab171974/Images/ab171974-194482-anti-tbp-1-antibody-epr12153-flow-cytometry.jpg)
Flow Cytometry - Anti-TBP-1 antibody [EPR12153] (ab171974)Flow cytometric analysis of permeabilized HeLa cells labeling TBP-1 with ab171974 at 1/100 dilution (red) or a Rabbit IgG (negative) (green).
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![Anti-TBP-1 antibody [EPR12153] (ab171974)](https://www.abcam.com/ps/products/171/ab171974/Images/ab171974-5-benefits-of-recombinant-antibodies.png)
Anti-TBP-1 antibody [EPR12153] (ab171974)
