Anti-TMS1/ASC antibody [EPR10403] (ab155970)
![Anti-TMS1/ASC antibody [EPR10403] (ab155970)](https://www.abcam.com/ps/products/155/ab155970/Images/ab155970-270898-anti-tms1-antibody-epr10403-western-blot.jpg "Anti-TMS1/ASC antibody [EPR10403] (ab155970)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10403] to TMS1/ASC
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-TMS1/ASC antibody [EPR10403]
See all TMS1/ASC primary antibodies -
Description
Rabbit monoclonal [EPR10403] to TMS1/ASC -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human
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Immunogen
Synthetic peptide within Human TMS1/ASC aa 100 to the C-terminus. The exact sequence is proprietary.
Database link: Q9ULZ3 -
Positive control
- WB: THP-1 and U937 cell lysates. ICC/IF: HeLa and THP-1 cells. Flow Cyt: THP-1 cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as TMS1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10403 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TMS1/ASC antibody [EPR10403] (ab155970)All lanes : Anti-TMS1/ASC antibody [EPR10403] (ab155970) at 1/5000 dilution
Lane 1 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
Lane 2 : U937 (Human histiocytic lymphoma cell line) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 20000 µg
Predicted band size: 22 kDaBlocking and diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-TMS1/ASC antibody [EPR10403] (ab155970)](https://www.abcam.com/ps/products/155/ab155970/Images/ab155970-163270-anti-tms1-antibody-epr10403-western-blot.jpg)
Western blot - Anti-TMS1/ASC antibody [EPR10403] (ab155970)All lanes : Anti-TMS1/ASC antibody [EPR10403] (ab155970) at 1/1000 dilution
Lane 1 : THP1 cell lysate
Lane 2 : U937 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 22 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody [EPR10403] (ab155970)](https://www.abcam.com/ps/products/155/ab155970/Images/ab155970-270899-anti-tms1-antibody-epr10403-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody [EPR10403] (ab155970)ab155970 staining TMS1/ASC in U937 (Human histiocytic lymphoma cell line) cells by ICC/IF (Immunocytochemistry/immunofluorescence).
Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab195889 was used as a counterstain for primary antibody ab155970 at 1/200. DAPI was used as a nuclear counterstain and PBS as a negative control.
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![Flow Cytometry - Anti-TMS1/ASC antibody [EPR10403] (ab155970)](https://www.abcam.com/ps/products/155/ab155970/Images/ab155970-270901-anti-tms1-antibody-epr10403-flow-cytometry.jpg)
Flow Cytometry - Anti-TMS1/ASC antibody [EPR10403] (ab155970)ab155970 staining TMS1/ASC in the human cell line THP-1 (Human monocytic leukemia cell line) by flow cytometry.
Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/50. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabeled control: Cell without incubation with primary antibody and secondary antibody (Blue)
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![Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody [EPR10403] (ab155970)](https://www.abcam.com/ps/products/155/ab155970/Images/ab155970-163269-anti-tms1-antibody-epr10403-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody [EPR10403] (ab155970)Immunofluorescence analysis of THP-1 (Human monocytic leukemia cell line) cells, labeling TMS1/ASC using ab155970 at a 1/100 dilution.
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![Anti-TMS1/ASC antibody [EPR10403] (ab155970)](https://www.abcam.com/ps/products/155/ab155970/Images/ab155970-7-benefits-of-recombinant-antibodies.png)
Anti-TMS1/ASC antibody [EPR10403] (ab155970)
