Human Neuro Antibody Array I (Membrane, 20 Targets) (ab211062)
Overview
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Product name
Human Neuro Antibody Array I (Membrane, 20 Targets) -
Sample type
Serum, Plasma, Other biological fluids, Cell Lysate, Cell culture media, Tissue Lysate -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Human Neuro Antibody Array I (ab211062) for use with cell culture media, serum, plasma, cell and tissue lysates and other liquid samples.
Targets: BDNF, beta-NGF, GCSF, GDNF, HB-EGF, IFN gamma, IGF-1, IL-10, IL-1 alpha, IL-1 beta, IL-6, IL-8, MCP-1, MIP-1 alpha, MMP-2, MMP-3, S100B, TGF beta, TNF alpha, VEGF-A.
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Notes
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1 mL of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
Learn more about cytokine arrays and other membrane antibody arrays
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Tested applications
Suitable for: Multiplex Protein Detectionmore details
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 2 Membrane 4 Membrane 8 Membrane 1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 1 x 50µl 20X Wash Buffer I 1 x 10ml 1 x 10ml 1 x 20ml 20X Wash Buffer II 1 x 10ml 1 x 10ml 1 x 20ml 2X Cell Lysis Buffer 1 x 10ml 1 x 10ml 1 x 16ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit Antibody Arrays 2 units 4 units 8 units Biotinylated Antibody Cocktail 1 vial 2 vials 4 vials 1X Blocking Buffer 1 x 25ml 1 x 25ml 2 x 25ml Detection Buffer C 1 x 1.5ml 1 x 1.5ml 1 x 2.5ml Detection Buffer D 1 x 1.5ml 1 x 1.5ml 1 x 2.5ml Plastic sheets 1 unit 1 unit 1 unit
Images
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Typical results
Membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at room temperature for 1 minute.
