Anti-NFkB p105 / p50 (phospho S337) antibody (ab28849)
Key features and details
- Rabbit polyclonal to NFkB p105 / p50 (phospho S337)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NFkB p105 / p50 (phospho S337) antibody
See all NFkB p105 / p50 primary antibodies -
Description
Rabbit polyclonal to NFkB p105 / p50 (phospho S337) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human NFkappa B p105/p50 around the phosphorylation site of Serine 337.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p105 / p50 (phospho S337) antibody (ab28849)Ab28849, at a dilution of 1/50, staining p105 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
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Western blot - Anti-NFkB p105 / p50 (phospho S337) antibody (ab28849)All lanes : Anti-NFkB p105 / p50 (phospho S337) antibody (ab28849)
Lane 1 : Untreated extracts from MDA-MB-435 cells (5-30ug).
Lane 2 : Extracts from MDA-MB-435 cells (5-30ug) treated with synthesized phosphopeptide.
Predicted band size: 106 kDa
Observed band size: 118 kDa why is the actual band size different from the predicted?
