Anti-NFkB p105 / p50 (phospho S337) antibody (ab194729)
Key features and details
- Rabbit polyclonal to NFkB p105 / p50 (phospho S337)
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-NFkB p105 / p50 (phospho S337) antibody
See all NFkB p105 / p50 primary antibodies -
Description
Rabbit polyclonal to NFkB p105 / p50 (phospho S337) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human NFkB p105/ p50 (phospho S337). The exact sequence is proprietary. Amino acid sequence corresponds to residues surrounding S337.
Database link: P19838 -
Positive control
- WB: TNF-a treated HeLa and C6 cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NFkB p105 / p50 (phospho S337) antibody (ab194729)All lanes : Anti-NFkB p105 / p50 (phospho S337) antibody (ab194729) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : C6 cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Developed using the ECL technique.
Predicted band size: 105 kDa
Exposure time: 1 secondHeLa cells were treated by TNF-α (20 ng/ml) and Calyculin A (100 nM) at 37°C for 10 minutes.
C6 cells were treated by TNF-α (20 ng/ml) at 37°C for 10 minutes.
Blocking buffer: 3% nonfat dry milk in TBST.
